hema labbbbbbb Flashcards
is a non-specific test that indicates changes in plasma protein concentrations due to infection or injury
Erythrocyte sedimentation rate (ESR)
The ESR test is performed in the laboratory by placing anticoagulated blood
upright tube (Westegren’s most often)
The initial lag phase
10m
The packing phase
10m
The phase of rapid RBC falling
40m
Methods of measuring esr
Westerngren Method
Wintrobe and Landsber method
The test measures sedimentation of red cells( in diluted blood) after one hour in an open ended tube mounted vertically on a stand.
Westerngren method
The recommended tube for westerngren method
straight glass or rigid plastic transparent tube
length in westerngren method
30 cm in length and not less than 2.55 mm in diameter.
Venous Blood is collected into a
sodium citrate tube or EDTA tube
The result is expressed as ESR
X mm in 1 hr
The test should be carried out within
4 hours of collecting the blood
A delay up to 6 hours is permissible provided that the blood is kept at
4 degree C.
EDTA blood can be used 24 hours on a specimen that was kept in 4 degree C if the volume of
109 mmol/l trisodium citrate is added to 4 volumes of blood
ESR is helpful in diagnosing two specific inflammatory diseases
temporal arteritis
polymyalgia rheumatica
ESR of more than 100 mm/hr is strongly associated with serious underlying disorders like
connective tissue disease
infections
malignancies
This method of cell counting was originally developed by Coulter Electronics and is referred to as the Coulter principle.
THE ELECTRICAL IMPEDANCE PRINCIPLE
is the most common light source used in flow cytometers
Laser light
Properties of flow cytometry
intensity
stability
monochromatism
Measures how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution.
spectrophotometry
Hemoglobin determination
Cyanmethemoglobin method
In a Wright-stained blood smear, young reticulocytes with a high amount of RNA residual have a blue appearance
polychromatophilia