a2.2- cell structure Flashcards
disadvantages of electron microscopy
- specimen needs to die
advantages of electron microscopy [2]
- higher range of magnification (can detect smaller structures)
- higher resolution
why electron microscopy have higher resolution
shorter wavelength
freeze fracture
cut in half → spray metal
- see inside of membrane
cryogenic electron microscopy
see proteins with atomic precision- can design drugs that fit into them
florescent stains
bright colours- see it clearer
immunofluorescence
tag antibodies
structures common to calls in all living organisms [2]
- dna as genetic material
- cytoplasm enclosed by plasma membrane composed of lipids
use of plasma membrane
separating interior form its surrounding
functions of cytoskeleton [3]
- maintain cell shape
- organises cell parts
- enables cells to move and divide
components of cytoskeleton
- microtubules (movement)
- actin filaments (movement)
- intermediate filaments (structural)
process of life in unicellular organisms
metabolism
reproduction
homeostasis
growth
response to stimuli
excretion
nutrition
difference between eukaryotes (animals, plant and fungi)
see table
endosymbiotic theory
- explains the existence of several organelles of eukaryotes
- originated as symbioses between separate single-celled organisms
- eukaryotic organisms engulfed by a prokaryotic cell, was not digested
- remained inside host cell, carrying out aerobic respiration and providing energy to their host cells, evolving into mitochondria.
what does evidence suggest about eukaryotes
- all eukaryotes evolved form common ancestor that had a nucleus and reproduced sexually
evidence of endosymbiotic theory [4]
MAD DRS
Membranes
- double membrane
- inner membrane has proteins similar to prokaryotes
Antibiotics
- susceptible to antibiotics (chloramphenicol)
- organelles may have bacterial origins
Division
- reproduction occurs via a fission-like process
- only through division of pre-existing mitochondria and chloroplasts
DNA
- own DNA: naked and circular
- like prokaryotic DNA structure
- transcribe their DNA
- use the mRNA to synthesise some of their own proteins
Ribosomes
- 70S
- similar to prokaryotes
Size
- similar size to bacteria
cell differentiation
- development of a cell to carry out specific function
- different patterns of gene expression: often triggered by changes in the environment