7.1 Laboratory ID of Lymphocytes

Question 1

Identify cells based on scattering of light as cells flow in a single file through a laser beam

Answer 1

Flow cytometry

Question 2

Flow cytometry

Cell size

a. Forward Light scatter
b. Side Light scatter

Answer 2

a

Question 3

Flow cytometry

Cell granulolarity

a. Forward Light scatter
b. Side Light scatter

Answer 3

b

Question 4

Use to identify T cell

a. flow cytometry
b. Fluorescence microscopy
c. Rosette assay
d. Density gradient Centrifugation

Answer 4

c

Question 5

In rosette assay, how can you tell if it is a T cell?

Answer 5

Sheep RBC surrounds the T cells forming like a “Rose” or in a Rosette formation

Question 6

In rosette formation, what CD marker helps bind Sheep RBC to T cell?

Answer 6

CD2 marker

Question 7

In rosette assay, B cell does not have CD2 marker that is why no Rosetter formation

T or F

Answer 7

T

Question 8

In Density gradient centrifugation:

has a density of 1.077

a. Ficoll Hypaque
b. Nylon Wool
c. Hanks PBS
d. RPM1-1640

Answer 8

a

Question 9

In Density gradient centrifugation:

B cells will adhere to this

a. Ficoll Hypaque
b. Nylon Wool
c. Hanks PBS
d. RPM1-1640

Answer 9

b

Question 10

T Cells may bind to Nylon wool

T or F

Answer 10

F

DO not bind

Question 11

In Density gradient centrifugation:

Washing, suspending and diluting medium

a. Ficoll Hypaque
b. Nylon Wool
c. Hanks PBS
d. RPM1-1640

Answer 11

c

Question 12

In Density gradient centrifugation:

Culture medium

a. Ficoll Hypaque
b. Nylon Wool
c. Hanks PBS
d. RPM1-1640

Answer 12

d