16.1 MHC/HLA typing

Question 1

a Phenotyping or genotyping identification of the HLA antigens or genes in a transplant candidate or donor

Answer 1

MHC/HLA typing

Question 2

Complement-dependent cytotoxicity

a. Phenotypic
b. Genotypic

Answer 2

a

Question 3

Mixed lymphocyte reaction

a. Phenotypic
b. Genotypic

Answer 3

a

Question 4

In Complement-dependent cytotoxicity:

If the cells POSSESS the ___ antigen defined by the antibody in that well, __________ is activated the cells are killed and this killed cells are able to take up the vital dye

Answer 4

HLA

Complement

Question 5

What is the vital dye for Complement-dependent cytotoxicity?

Answer 5

Trypan blue

Question 6

What is the positive result for Mixed Lymphocyte reaction?

Answer 6

Blastogenesis Reaction / Mixed lymphocyte reaction

Question 7

In Blastogenesis Reactions

A cellular response showing lymphocytes from ___ individuals to undergo Blast transformation when cultured In _____

Answer 7

2

Vitro

Question 8

Genotypic:

A based amplification of HLA genes followed by analysis of the amplified DNA to identify the specific HLA allele or allele group

Answer 8

PCR

Question 9

Gold standard for HLA typing

Answer 9

Sequence-based typing

Question 10

Which of the following does not belong for HLA PCR:

PCR with sequence-specific PCR

PCR-sequence-specific oligonucleotide probe hybridization

Answer 10

None