Topic 4 (enzyme health) Flashcards
Enzymes involved in diagnostic markers (liver)
ALT / AST
Enzymes involved in diagnosis tests (glucose)
GDH / GOX
What do enzymes DONT do
Doesnt shift position of equilibrium
Rate of reaction is based off what?
Determined by height of activation barrier
How does Enzymes decrease activation energy (3)
Provides catalytically competent groups for specific reaction
Binds substrate so its optimised for reaction
Binds/stabilises transition state of reaction
At active site, what is the mechanism of binding
Binds to substrate w/multiple weak interactions
Provides substrate specificity
Michaelis constant equation:
km = K2 + K3
- - - - - -
K1
Formation of product (V) equation:
V = S
- - - - - - - -
S + Km
Factors affecting reaction rates (5)
Substrate concentration Enzyme concentration pH Temperature Inhibitors
What type of inhibition binds to side chains AA at active site leading to covalent modification of enzyme
Irreversible Inhibition
Name a drug that leads to carboxymethylation of cysteine chains
Iodoacetamide
What drug alters serine residues of AChE
Nerve Agents
With enzyme kinetics, what happens to V(max) and Km in irreversible inhibition?
Km + Vmax Decrease
What type of inhibition can be overcome by high substrate concentration?
Competitive Inhibition
Competitive inhibition enzyme kinetics?
Vmax Unchanged
Km Increased
What type of inhibition binds to an independent site of enzyme not Active site?
Non competitive
Enzyme kinetics of non competitive
Vmax decreased
Km unchanged
Methotrexate + Dihydrofolate Reductase reaction is what sort of inhibition?
Competitive Irreversible Reaction
Non steroidal anti inflammation prevents creation of what?
Prostaglandins
Aspirin modifies what?
What sort of inhibition is this?
What enzyme does it bind?
Serine residues via covalent bond
Competitive irreversible inhibition
COX-1
Ibuprofen is what sort of reaction?
What enzyme does it bind?
Competitive reversible inhibition
COX-1 + COX-2
