SA Ortho - Regenerative Med Flashcards
Factors of regenerative medicine
Scaffold = delivery vehicles & matrix scaffold
Cells
Bioactive factors
Extracellular matrix role
Dual function proteins
- structural and functional
Controlled released of growth factors
Degradation results in release of biologically active proteins
Tropic mediators
Bioactive factors
- growth factors
- cytokines
functions of bioactive factors
Recruit endogenous cells
Inhibit apoptosis
Inhibit fibrosis
Stimulate angiogenesis
Immunomodulation
Anti-inflammatory
Anti pain
Autologous bone marrow
Stem cells + growth factors
Bone marrow derived cells
Cells are collected, cultured and then injected into tendon area - note that injections into areas/space created by lesions are ideal
Adipose tissue derived cells
Fat has more adult stem cells per unit than bone marrow
Ship to company to process - chemical digest and centrifuge to isolate the stem cells
Rapid return for injection
Stromal vascular fraction =
Nucleated cells
Fibroblastic cells
Pericytes
Immune cells
Vascular smooth muscle cells
Endothelial cells
Heterogenaic
Mixed function cells
Bone marrow derived cell summary
Expand in vitro
Adipose tissue
Kinds of bioactive factors
Platelet rich plasma
Bone marrow aspirate concentrate
Autologous conditioned serum
Blood products
Autologous
Specimen is taken from subject and put pack in the the same subject
Platelet rich plasma
Multiple growth factors (IGF-I, TGF-B, PDGF- BB)
Bioactive scaffold fibrin - turns into fibrinogen
Relatively inexpensive
Autologous condition serum
Point of care (24 hr)
Growth factors + “IRAP”
Relatively inexpensive
