Poxviridae - Diagnosis Flashcards
1
Q
Where should sampling material be collected?
A
Scrapings from skin lesions, vesicular fluid, crusts, scabs
2
Q
How can you tell Orthopoxviruses from Parapoxviruses?
A
Ortho- Brick shaped
Para- Ovoid
(slide 54 photo)
3
Q
What types of inclusion bodies should you look for in histopathology?
A
Type B/Guarnieri: Slightly basophilic, composed of viral particles, and protein aggregates. Seen in most poxviruses.
Type A/ATI: Cowpox and ectromelia virus induce these. Strongly eosinophilic.
Bollinger and Borrel bodies in avipoxvirus.
4
Q
Where would you inoculate an egg with poxvirus?
A
Chorioallantoic membrane (CAM) and look for pock lesions. **PARAPOXVIRUSES do NOT multiply in chicken embryo**
5
Q
What other methods can you use to detect Poxviruses?
A
ELISA serological assay
PCR
