Poxviridae - Diagnosis

Question 1

Where should sampling material be collected?

Answer 1

Scrapings from skin lesions, vesicular fluid, crusts, scabs

Question 2

How can you tell Orthopoxviruses from Parapoxviruses?

Answer 2

Ortho- Brick shaped
Para- Ovoid
(slide 54 photo)

Question 3

What types of inclusion bodies should you look for in histopathology?

Answer 3

Type B/Guarnieri: Slightly basophilic, composed of viral particles, and protein aggregates. Seen in most poxviruses.

Type A/ATI: Cowpox and ectromelia virus induce these. Strongly eosinophilic.

Bollinger and Borrel bodies in avipoxvirus.

Question 4

Where would you inoculate an egg with poxvirus?

Answer 4

Chorioallantoic membrane (CAM) and look for pock lesions.
**PARAPOXVIRUSES do NOT multiply in chicken embryo**

Question 5

What other methods can you use to detect Poxviruses?

Answer 5

ELISA serological assay

PCR