Adenoviridae - General Characteristics Flashcards
What is the morphology of adenoviridae?
Non-enveloped
Hexagonal
Icosahedral symmetry
70-90 nm in diameter
Capsid - 720 herons arranged as 240 trimers
12 pentons with a fiber that protrude from the surface
What does the genome look like?
Non-segmented
Linear
Double-stranded DNA
35-36kb
Where does replication take place?
Nucleus
How are virions released?
Cell lysis
When tips of the penton fibers bind to surface receptors on RBC’s, what happens?
Hemagglutination
T/F Some adenoviridae are oncogenic in laboratory animals.
True.
What are adenoviruses inactivated easily by?
Common disinfectants
Can adenoviruses infect more than one species?
Yes but they have narrow host ranges
What pathogenesis do adenoviruses cause?
Acute respiratory disease
Gastroenteric disease
(mostly subclinical)
Which proteins of adenoviruses are toxic to the cell?
Penton and fiber proteins
What protein inhibits MHCI antigen transport?
E3/19K
TNF-induced apoptosis is inhibited by which protein?
E3/14.7K
How do they modulate antiviral inflammatory responses?
Inhibit nuclear factor kB transcriptional activity
Where do AV’s persist in long periods of latency?
Lymphoid tissues, tonsils, adenoids, and PP
Reactivated in immunocompromised animals
What type of animals is AV highly pathogenic in?
Immunodeficient animals
Which gene products produced by AV make them oncogenic?
E1A: Inactivate Rb protein
E1B: Inactivate p53 (so defective DNA isnt put through apoptosis)
How many genera are there? Which are important?
There are 4.
Two important ones:
Mastadenovirus (animal)
Aviadenovirus (birds)
How many penton fibers project from Mastadenovirus?
One.
How many penton fibers project from Aviadenovirus?
One but it is bifurcated so it appears as two.
