(Part 2) 2.8.2 G. vaginalis Microscope, Culture, and Biochemical test Flashcards
G. vaginalis can be usually observed in wet mounts when BV is suspected
T or F
T
These are large squamous epithelial cells with G+ on the edges
This is usually known as Dirty looking squamous epithelial cells cause by G. vaginalis
Clue cells
What is the most common specimen for G. vaginalis?
Vaginal discharge
G. vaginalis grows best at ____ CO2 and a Temp at________
5-7%, 35-37C
Cultural characteristic of G. vaginalis is similar to Eryisipelothrix which is?
Nonhemolytic, pinopoint colony
What is the medium of choice for G. vaginalis
Human blood bilayer tween agar
In human blood bilayer tween agar, what prevents growth of yeasts and fungi?
CNA agar and Amphoteicin B
What is the purpose of Human blood in Human blood bilayer tween agar?
enhance beta hemolysis
Catalase Neg or Pos for G. vaginalis?
Neg
Oxidase neg or pos for G. vaginalis
Neg
Hippurate hydrolysis for G. vaginalis
Neg or pos
Pos
Most common non cultural test for detection of G. vaginalis
KOH test
KOH test is known as
Whiff test
How is KOH test is done?
Vaginal secretion + 10% KOH
Positive result for KOH test of G. vaginalis
Pungent, Fishy amine-like odor