(Part 1) 1.15 Other microbiological stains

Question 1

Fluorochrome dye which binds to the nucleic acid and produce bright orange fluorescence

Answer 1

Acridine orange

Question 2

Uses Tannic acid and a dye to stain flagella

Answer 2

Leifson stain

Question 3

Use for staining endospore,

Answer 3

Sachaeffer fulton

Question 4

What is being stained in Acridine orange?

Answer 4

Nucleic acid

Question 5

What is the primary stain for Sachaefer Fulton

Answer 5

Malachite Green

Question 6

What is the counter stain for Sachaefer Fulton

Answer 6

Safranin

Question 7

Traditionally used to stain C. diphtheriae for observation of metachromatic granules

Answer 7

Methylene blue

Question 8

Stain for bipolar bodies

Answer 8

Wayson stain

Question 9

Fluorochrome that binds to chitin in fungal cell walls; fluoresces as apple green or blue white

Answer 9

Calcofluor white

Question 10

Used to stain the cell wall of medically important fungi grown in side culture

Answer 10

Lactophenol blue

Question 11

Negative stain used to visualize capsules surrounding certain yeast

Answer 11

India ink

Question 12

What is the specific fungi being detected in india ink?

Answer 12

Cryptococcus neoformans

Cryptococcus spp.