(Part 2) 2.2.3 Strep Media and Test Flashcards
Which of the following cant grow Strep spp.?
SBA
CHOC
PEA
CNA
None
Abiotrophia and Granulicatella cannot grow in SBA
T or F
T
Abiotrophia and Granulicatella requires what vitamin to be grown?
Vitamin B6
Used for Isolating GAS from Throat Swabs? (Full name)
5% Sheep blood Agar
w/
Trimetophrim-Sulfamethoxazole (SXT)
LIM broth isolates what strep?
Group B strep
LIM broth is a Todd-Hewitt broth that contains?
CNA (Colistin Nalidixic acid)
A selective - Differential based on esculing hydrolysis that isolates enterococci?
Enterococcosel agar
What is the Sus ZOI for Taxo P?
What is Taxo P?
> 14mm
Optochin
What is the Sus ZOI for Taxo A
What is Taxo A?
> 10mm
Bacitracin
What is the reagent of PYR?
Pyrolidonyl-Naphthylamide
What is the positive indicator for PYR?
Bright Red
What is the meaning of CAMP?
Christie-Atkins-Munch-Peterson
What is the Streaked organism (Control) used for CAMP [Vertical]?
S. aurues
What is the result for CAMP?
Arrowhead
CAMP is used to determine what strep?
S. agalactiae
S. aureus act as a source of ______ in CAMP
Beta lysin
Enhance Beta lysin for Group B
What media is used for CAMP?
BAP
What is the reagent for Hippurate hydolysis
Hippuric acid / Benzoic acid
What happens to hippuric acid when is being hydrolysed
Hippric acid -> glycine
Positive indicator for Hippurate hydrolysis
Purple
What strep is positive for Hippurate hydrolysis?
Strep agalactiae
What is the inhibitor used in Bile esculing hydrolysis?
40% bile
In bile esculin, what is used to differentiate Enterococci to other strep?
Esculin
What is the positive indicator for Bile esculin?
Blackening of agar
What is the reagent for Salt tolerance test?
6.5% NaCl broth
What is the positive indicator for Salt tolerance test?
Turbidity
What is the reagent used for Bile solubility test?
Na deoxycholate and Na taurocholate
What is the positive indicator for Bile solubility test?
Lysis of colonies
What is the reagent for Optochin test
Ehtylhydrocuprene hydrochloride
Size and grams of Ethylhydrocuprene hydrochloride?
6mm, 5ug disk
What bacteria is positive in Optochin?
S. pneumoniae
What bacteria is positive in Bile solubility?
S. pneumoniae
This has a positive result for Capsular swelling?
Antiserum for capsule
Neufeld reaction (Quellung reaction)
S. pneumoniae has capsule
T or F
T