(Part 2) 2.1.1 Test and Media for Staph and Micrococcus Flashcards
Staph and Micrococcus are Catalase Positive
T or F
T
Only Staph can grow in an aerobic environment while micrococcus not
T or F
F
Micrococcus can grow in an Anaerobic environment
T or F
F
Obligate aerobe
Staph can oxidize Glucose
T or F
F
Ferment
Micrococcus can’t ferment glucose
T or F
T
Bacteria that have a result of Blue-Purple in Microdase test
Micrococcus
Positive in benzidine?
Staph or micro
Micro
Sus in Lysostaphin?
Staph or micro
Staph
Res in Taxo A
Staph or micro
Staph
Sus in Lysostaphin
Staph or Micro
Staph
What is the reagent used in Catalase test for Staph and micrococcus
3% Hydrogen Peroxide (H2O2)
What is the reagent for Microdase test / Modified oxidase test
Tetramethyl-Phenylenediamene-Dihydrochloride in a DimethylSulfoxide
(TMPD in a DMSO)
What is Positive result for Catalase test?
Vigorous bubbling
What is the Positive result for Microdase Test
BLue-Purple/BLue
What is the principle for Catalase test?
Catalase enzyme hydrolyzes H2O2 in to Water (H2O) and Oxygen (O2) which result to bubbling