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MTAP 1 Bacte > (Part 2) 2.1.1 Test and Media for Staph and Micrococcus > Flashcards

(Part 2) 2.1.1 Test and Media for Staph and Micrococcus Flashcards

1
Q

Staph and Micrococcus are Catalase Positive

T or F

A

T

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2
Q

Only Staph can grow in an aerobic environment while micrococcus not

T or F

A

F

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3
Q

Micrococcus can grow in an Anaerobic environment

T or F

A

F

Obligate aerobe

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4
Q

Staph can oxidize Glucose

T or F

A

F

Ferment

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5
Q

Micrococcus can’t ferment glucose

T or F

A

T

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6
Q

Bacteria that have a result of Blue-Purple in Microdase test

A

Micrococcus

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7
Q

Positive in benzidine?

Staph or micro

A

Micro

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8
Q

Sus in Lysostaphin?

Staph or micro

A

Staph

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9
Q

Res in Taxo A

Staph or micro

A

Staph

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10
Q

Sus in Lysostaphin

Staph or Micro

A

Staph

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11
Q

What is the reagent used in Catalase test for Staph and micrococcus

A

3% Hydrogen Peroxide (H2O2)

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12
Q

What is the reagent for Microdase test / Modified oxidase test

A

Tetramethyl-Phenylenediamene-Dihydrochloride in a DimethylSulfoxide

(TMPD in a DMSO)

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13
Q

What is Positive result for Catalase test?

A

Vigorous bubbling

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14
Q

What is the Positive result for Microdase Test

A

BLue-Purple/BLue

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15
Q

What is the principle for Catalase test?

A

Catalase enzyme hydrolyzes H2O2 in to Water (H2O) and Oxygen (O2) which result to bubbling

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16
Q

What is the Medium for DNase test?

A

DNA methyl green complex

17
Q

What is the result for DNase test?

A

Clearing of Medium

18
Q

What is the principle for DNase test?

A

The ability of the bacteria to hydrolyzes DNA medium complex which results to clearing

19
Q

SBA and CHOC agar can be used to grow Staph?

T or F

20
Q

What are the selective medium for Staph?

A

PEA and CNA agar

21
Q

What is the Selective-Differential media for Staph?

A

Mannitol salt agar

22
Q

What is the inhibitor for MSA?

23
Q

What is the component of MSA that differentiates Staph?

24
Q

What is the pH indicator for MSA/

A

Phenol Red

25
Q

Positive indicator for MSA when it comes to differential??

A

Yellow color

26
Q

Can Staph epidermidis and S. saprophyticus grow in MSA

27
Q

What Staph ferments Mannitol?

28
Q

Which among the staph is Halophilic?

29
Q

What is the Method for Slide coagulase and tube coagulase

A

Slide: Slide test
Tube: Tube Test

30
Q

What is being detected in Slide coagulase?

A

Bound coagulase / CLotting factr

31
Q

What is being detected in Tube method?

A

Free Coagulase

32
Q

What is the reagent for SLide and Tube?

A

Rabbit plasma

33
Q

What is the result for Slide test?

A

Clump (30sec)

34
Q

What is the result for Tube method?

A

Clot formation when incubated in 35C for 4 hrs

MTAP 1 Bacte (93 decks)

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