(Part 1) 1.18 Specimen management Flashcards
Specimens should be collected during ___________ of an illness and before antimicrobials, antifungals, or antiviral medications are administered, if possible
acute phase/ early stage
within how many mins/hrs should sterile body fluids such as CSF and other body fluids should be transported to the lab?
15 mins
within how many mins/hrs should stool and urine be transported to the lab?
2 hrs
All specimen containers should be leak-proof, and the specimens should be transported within sealable leak-proof, plastic bags with a separate section for paperwork
T or F
T
Specimen integrity includes?
Correct modes of COLLECTION
HANDLING of specimen
PRESERVATION
time of TRANSPORT
Specimen that should be stored in 4 C
Urine
Stool
Sputum
Swab
Catheter tips
(youSSS see) USSSC
Specimens that should be stored in 25 C
CSF
Body fluids (Serous fluids)
IV catheters
Abscess
Gastric aspirate
Genital specimen
Throat/Nasopharyngeal
Specimens that should be stored in 35 C
CSF (Hold only for 6 hrs)
Blood is drawn at time of _______ episode
Febrile
How many sample of blood should be taken?
2-3 samples
Blood Volume for adults and pedia?
Adults: 20 mL
Pediatric: 1-20mL
Anticoagulant used in blood?
This inhibits phagocytosis (Neutralizes bactericidal effect in human serum)
0.025% SPS (Sodium Polyanethol Sulfonate)
Routine blood culture is done every?
5-7 days
How sputum is collected?
Deep cough
how to determine the sputum suitability fr gram staining?
<10 epithelial cells / LPF
>25 PMNS / LPF
> 10 epithelial cells / LPF
<25 PMNS / LPF
is this suitable?
no, the specimen is saliva
How to collect urine?
Midstream catch
How to collect urine if the specimen requires anaerobically?
Suprapubic Urine
How to collect catheterized urine specimens?
First 15mL discard then the remaining collect
Preservative used for urine?
Boric Acid
Major cause of ascending UTI?
Escherichia coli
What bacteria is to be expected if stool samples is received?
Salmonella and Shigella, Campylobacter
What is the transport media used for stool and rectal swab if the transportation exceeds 1 hr?
Cary Blair medium
How to collect rectal swab?
1-1.5 cm past anal sphincter
What tube # is being collected in CSF?
Tube 2
How to store CSF?
35C for 6hrs
If only one tube of CSF is received, what is the chronological order of lab to receive csf specimen?
1st Microbiology
2nd Hematology
3rd Chemistry and Serology
What specimen is used for routine culture of Group A Streptococcus
Throat/Pharynx
Specimen of choice for Bordetella pertusis?
Nasopharynx
How to collect nasopharynx?
Similar to collecting COVID 19 specimen swab and rotate for 5 secs in nasopharynx
NOTE: (Superficial abscess)
* Wipe area with sterile saline or 70% alcohol
* Aspirate if possible, swab along leading edge of wound
Most important in specimen labeling
Complete patient’s name
incubation Temp for Fungi?
28 - 30C
Incubation temp for most bacteria, viruses and acid fast bacillus?
35-37C
35C more optimum bacterial growth
21% oxygen
0.03% CO2
Aerobes
5-10% CO2
15% Oxygen
Capnophiles
5-10% Oxygen
8-10% CO2
Microaerophiles
- Do not require oxygen
- 5% to 10% hydrogen
- 5% to 10% CO2
- 80% to 90% nitrogen
- 0% O2
Anaerobes
