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MTAP 1 Bacte > (Part 1) 1.18 Specimen management > Flashcards

(Part 1) 1.18 Specimen management Flashcards

1
Q

Specimens should be collected during ___________ of an illness and before antimicrobials, antifungals, or antiviral medications are administered, if possible

A

acute phase/ early stage

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2
Q

within how many mins/hrs should sterile body fluids such as CSF and other body fluids should be transported to the lab?

A

15 mins

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3
Q

within how many mins/hrs should stool and urine be transported to the lab?

A

2 hrs

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4
Q

All specimen containers should be leak-proof, and the specimens should be transported within sealable leak-proof, plastic bags with a separate section for paperwork

T or F

A

T

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5
Q

Specimen integrity includes?

A

Correct modes of COLLECTION

HANDLING of specimen

PRESERVATION

time of TRANSPORT

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6
Q

Specimen that should be stored in 4 C

A

Urine
Stool
Sputum
Swab
Catheter tips

(youSSS see) USSSC

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7
Q

Specimens that should be stored in 25 C

A

CSF
Body fluids (Serous fluids)
IV catheters
Abscess
Gastric aspirate
Genital specimen
Throat/Nasopharyngeal

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8
Q

Specimens that should be stored in 35 C

A

CSF (Hold only for 6 hrs)

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9
Q

Blood is drawn at time of _______ episode

A

Febrile

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10
Q

How many sample of blood should be taken?

A

2-3 samples

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11
Q

Blood Volume for adults and pedia?

A

Adults: 20 mL
Pediatric: 1-20mL

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12
Q

Anticoagulant used in blood?

This inhibits phagocytosis (Neutralizes bactericidal effect in human serum)

A

0.025% SPS (Sodium Polyanethol Sulfonate)

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13
Q

Routine blood culture is done every?

A

5-7 days

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14
Q

How sputum is collected?

A

Deep cough

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15
Q

how to determine the sputum suitability fr gram staining?

A

<10 epithelial cells / LPF
>25 PMNS / LPF

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16
Q

> 10 epithelial cells / LPF
<25 PMNS / LPF

is this suitable?

A

no, the specimen is saliva

17
Q

How to collect urine?

A

Midstream catch

18
Q

How to collect urine if the specimen requires anaerobically?

A

Suprapubic Urine

19
Q

How to collect catheterized urine specimens?

A

First 15mL discard then the remaining collect

20
Q

Preservative used for urine?

A

Boric Acid

21
Q

Major cause of ascending UTI?

A

Escherichia coli

22
Q

What bacteria is to be expected if stool samples is received?

A

Salmonella and Shigella, Campylobacter

23
Q

What is the transport media used for stool and rectal swab if the transportation exceeds 1 hr?

A

Cary Blair medium

24
Q

How to collect rectal swab?

A

1-1.5 cm past anal sphincter

25
Q

What tube # is being collected in CSF?

A

Tube 2

26
Q

How to store CSF?

A

35C for 6hrs

27
Q

If only one tube of CSF is received, what is the chronological order of lab to receive csf specimen?

A

1st Microbiology
2nd Hematology
3rd Chemistry and Serology

28
Q

What specimen is used for routine culture of Group A Streptococcus

A

Throat/Pharynx

29
Q

Specimen of choice for Bordetella pertusis?

A

Nasopharynx

30
Q

How to collect nasopharynx?

A

Similar to collecting COVID 19 specimen swab and rotate for 5 secs in nasopharynx

31
Q

NOTE: (Superficial abscess)
* Wipe area with sterile saline or 70% alcohol
* Aspirate if possible, swab along leading edge of wound

A
32
Q

Most important in specimen labeling

A

Complete patient’s name

33
Q

incubation Temp for Fungi?

A

28 - 30C

34
Q

Incubation temp for most bacteria, viruses and acid fast bacillus?

A

35-37C

35C more optimum bacterial growth

35
Q

21% oxygen
0.03% CO2

A

Aerobes

36
Q

5-10% CO2
15% Oxygen

A

Capnophiles

37
Q

5-10% Oxygen
8-10% CO2

A

Microaerophiles

38
Q
  • Do not require oxygen
  • 5% to 10% hydrogen
  • 5% to 10% CO2
  • 80% to 90% nitrogen
  • 0% O2
A

Anaerobes

MTAP 1 Bacte (93 decks)

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