(Part 2) 2.21.3 Borrelia test and medium Flashcards
What is the specimen of choice for direct detection causing relapsing fever?
Peripheral blood
Organisms can be found in 70% of cases when examining blood specimens from febrile patients, using wet mount or thru the use of Giemsa or Wright’s stain (thick and thin blood smear)
T or F
T
What stain can be used to visualize B. burgdorferi?
a. Giemsa stain
b. Wright Stain
c. Stephen-John blue Stain
d. Warthin-Starry silver stain
d
PCR has become important in diagnosing Lyme disease
T or F
T
Borrelia are difficult to culture in vivo
T or F
F
In vitro
Modified kelly’s medium is also known as Barbour-Stoenner
Kelly Medium (BSK-II)
T or F
T
The best specimens for culture in untreated patients include the peripheral area of the EM ring lesion or synovial tissue
T or F
T
The CDC recommends a two-step approach to the serologic diagnosis of Lyme Disease
What is the first step?
Screen test (ELISA or IFA)
The CDC recommends a two-step approach to the serologic diagnosis of Lyme Disease
What is the 2nd step?
Confirmatory (Western blot)