(Part 2) 2.22.3 Clostridium Flashcards
Clostridium Endospores are for aerobically
T or F
F
Anaerobically
Clostridium is Gram?
Pos
Clostridium is a normal flora
T or F
T
Produces an extremely potent neurotoxin (BoNT)
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
b
[Botulinum neurotoxin]
Produces up to sevn exotoxins, including lethal toxin (LT), Hemorrhagic Toxin (HT) and enterotoxin A, B, C
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
c
Produces toxin A (TcdA) which is an enterotoxin, and toxin B (TcdB) which is a cytotoxic
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
e
Produces several exotoxins:
alphatoxin - destroys host cell membrane
Enterotoxin - insert and disrupts membrane
Beta toxin - cytotoxin
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
d
Produces a GTP (Glycosylate guanosine triphosphate which signals proteins to breakdown cellular cytotoxin and cell death
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
e
Produces tetanospasmin (TeNT), a neurotoxic exotoxin that disrupts nerve impulses to muscles
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
a
Causes tetanus
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
a
Commonly known as lockjaw
Tetanus
Causes foodborne botulism, Infant botulism, wound botulism, and intestinal botulism
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
b
Foodborne botulism colonizes GI tract of infants
T or F
F
Foodborne botulism is a flaccid paralysis (Rag doll)
Type of botulism associated with surgery and antibiotics
Intestinal botulism
Causes gas gangrene, necrotizing fasciitis, food poisoning, and enteritis necroticans
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
d
Causes Gas gangrene of the uterus as a result of abortion, normal , delivery, or cesarean section
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
c
This organism requires diminution of normal gut microbiota by the activity of various antimicrobial agents
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
e
Causes antibiotic-associated diarrhea and Pseudomembranous colitis
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
e
The only terminally located endospore in clostridium
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
a
Clostridium tetani is also known as?
Drumstick/Tackhead bacillus
Terminally = Endospore are at the end of bacillus
Clostridium perfringens endospores are rarely observed
T or F
T
What is the modified version of Lecithinase test
A. Neufeld Reaction
B. Nagler Reaction
c. Niger Reaction
d. Neufold Reaction
b
Causes the double zone of inhibition
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
d
Inner zone = Beta lysis (Theta toxin)
Outer zone = Alpha toxin
T or F
T
In reverse CAMP test, what is the streak organism/Control organism
a. S. agalactiae
b. Staph. aureus
c. Clostridium perfringens
d. Listeria monocytogenes
a
Positive in Reverse CAMP?
a. C. tetani
b. C. botulinum
c. C. sordelii
d. C. perfringens
e. C. difficile
d
Positive indicator for Lipase test?
Iredescent sheen when held at an angle to a light source
CCFA Medium (Cycloserin-Cefoxitin Fructose agar
Cycloserine- inhibits G(-)
Cefoxitin - inhibits G (+) and (-) but C. difficile
T or F
T
in CCFA, Colonies of C. difficile is describe?
Yellow ground glass
