(Part 2) 2.3.2 Media and Test for Neisseria Flashcards
This Neisseria only grows on Chocolate agar?
N. gonorrhoeae
What media grows N. meninigitidis
CHOC and 5% SBA
The priniciple of this test is to detect cytochrome C
Oxidase test
Reagent for Oxidase test ?
Tetramethyl-Phenylenediamene-Dihydrochloride
The use of nickel base alloy wires containing chromium and iron (Nicrom) to rub the colony paste on to the paper may result to False negative in Oxidase test
T or F
F
False positive
Positive indicator for Oxidase test?
Dark purple
What is the reagent for Superoxol catalase test?
30% hydrogen peroxide
Positive indicator for Catalase test?
Bubbling
Ability to utilize acetate?
Acetate utilization
All selective media for Neisseria contains vancomycin
T or F
T
All selective media fr Neisseria contains Colistin
T or F
T
In the selective media for Neisseria, this inhibits other gram (-)
Colistin
In the selective media for Neisseria, this inhibits Gram (+)
Vancomycin
All Selective media for Neisseria has Trimetophrim lactate?
T or F
F
Thayer martin does not have it
Selective media for neisseria that contains:
- Vancomycin
- Colistin
- Nystatin
Thayer Martin
Selective media for neisseria that contains (CHOC):
- Vancomycin
- Colistin
- Nystatin
- Trimetophrim Lactat
Modified Thayer martin
Selective media for neisseria that contains:
- Vancomycin
- Colistin
- Anisomysin
- Trimetophrim Lactat
Martin Lewis
Selective media for neisseria that contains:
- Vancomycin / Lincomycin
- Colistin
- Amphotericin B
- Trimetophrim Lactat
GC-Lect agar
Selective media for neisseria that contains (HORSE):
- Vancomycin
- Colistin
- Nystatin
- Trimetophrim Lactat
New York City
What is the base media for Thayer Martin?
CHOC
What is the base media for Martin Lewis
CHOC
What is the base media for New York City?
Lyse Horse blood and Horse Plasma
Which of the selective media is commercial media?
GC Lect agar
Reagent use to inhibit molds on Martin lewis?
Anisomycin
Transport media that is a flat bottle acted with MTM and a bottle under increased CO2
Transgrow bottles
Transport media that contains Flat plastic dish containing a medium selective for gonococcus and a tablet that acts as CO2 generator
JEMBEC Plate
John E. Martin Biological Environmental System
Determine by inoculating an extremely heavy suspension of the organisms to be tested in a small volume buffered, low peptone substrate with the appropriate carbohydrate
Carbohydrate utilization test
How many hrs for incubation of Carbohydrate utilization test
4 hrs
Carbohydrate for N. gonorrhoeae?
Glucose only
Carbohydrate for N. Meningitidis?
G, Maltos
Carbohydrate for N. Lactamica?
G, M, Lactose
Reagent use to inhibit molds on GC-LECT?
Amphotericin B
Which selective media can be used in Transgrow media?
Modified Thayer Martin
In selective media, what is reagent to inhibit swarming of proteus?
Trimetophrim Lactate
Reagent use to inhibit molds on Thayer martin, MTM, and NYCA?
Nystatin
What is the positive indicator for Carbohydrate utilization test?
Yellow color
(-) Red
DNase (+)
CUT (-)
Hockey puck colony
Moraxella catarrhalis
