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MTAP 1 Bacte > (Part 2) 2.3.2 Media and Test for Neisseria > Flashcards

(Part 2) 2.3.2 Media and Test for Neisseria Flashcards

1
Q

This Neisseria only grows on Chocolate agar?

A

N. gonorrhoeae

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2
Q

What media grows N. meninigitidis

A

CHOC and 5% SBA

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3
Q

The priniciple of this test is to detect cytochrome C

A

Oxidase test

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4
Q

Reagent for Oxidase test ?

A

Tetramethyl-Phenylenediamene-Dihydrochloride

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5
Q

The use of nickel base alloy wires containing chromium and iron (Nicrom) to rub the colony paste on to the paper may result to False negative in Oxidase test

T or F

A

F

False positive

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6
Q

Positive indicator for Oxidase test?

A

Dark purple

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7
Q

What is the reagent for Superoxol catalase test?

A

30% hydrogen peroxide

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8
Q

Positive indicator for Catalase test?

A

Bubbling

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9
Q

Ability to utilize acetate?

A

Acetate utilization

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10
Q

All selective media for Neisseria contains vancomycin

T or F

A

T

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11
Q

All selective media fr Neisseria contains Colistin

T or F

A

T

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12
Q

In the selective media for Neisseria, this inhibits other gram (-)

A

Colistin

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13
Q

In the selective media for Neisseria, this inhibits Gram (+)

A

Vancomycin

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14
Q

All Selective media for Neisseria has Trimetophrim lactate?

T or F

A

F

Thayer martin does not have it

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15
Q

Selective media for neisseria that contains:
- Vancomycin
- Colistin
- Nystatin

A

Thayer Martin

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16
Q

Selective media for neisseria that contains (CHOC):
- Vancomycin
- Colistin
- Nystatin
- Trimetophrim Lactat

A

Modified Thayer martin

17
Q

Selective media for neisseria that contains:
- Vancomycin
- Colistin
- Anisomysin
- Trimetophrim Lactat

A

Martin Lewis

18
Q

Selective media for neisseria that contains:
- Vancomycin / Lincomycin
- Colistin
- Amphotericin B
- Trimetophrim Lactat

A

GC-Lect agar

19
Q

Selective media for neisseria that contains (HORSE):
- Vancomycin
- Colistin
- Nystatin
- Trimetophrim Lactat

A

New York City

20
Q

What is the base media for Thayer Martin?

A

CHOC

21
Q

What is the base media for Martin Lewis

A

CHOC

22
Q

What is the base media for New York City?

A

Lyse Horse blood and Horse Plasma

23
Q

Which of the selective media is commercial media?

A

GC Lect agar

24
Q

Reagent use to inhibit molds on Martin lewis?

A

Anisomycin

25
Q

Transport media that is a flat bottle acted with MTM and a bottle under increased CO2

A

Transgrow bottles

26
Q

Transport media that contains Flat plastic dish containing a medium selective for gonococcus and a tablet that acts as CO2 generator

A

JEMBEC Plate

John E. Martin Biological Environmental System

27
Q

Determine by inoculating an extremely heavy suspension of the organisms to be tested in a small volume buffered, low peptone substrate with the appropriate carbohydrate

A

Carbohydrate utilization test

28
Q

How many hrs for incubation of Carbohydrate utilization test

A

4 hrs

29
Q

Carbohydrate for N. gonorrhoeae?

A

Glucose only

30
Q

Carbohydrate for N. Meningitidis?

A

G, Maltos

31
Q

Carbohydrate for N. Lactamica?

A

G, M, Lactose

32
Q

Reagent use to inhibit molds on GC-LECT?

A

Amphotericin B

32
Q

Which selective media can be used in Transgrow media?

A

Modified Thayer Martin

32
Q

In selective media, what is reagent to inhibit swarming of proteus?

A

Trimetophrim Lactate

33
Q

Reagent use to inhibit molds on Thayer martin, MTM, and NYCA?

A

Nystatin

34
Q

What is the positive indicator for Carbohydrate utilization test?

A

Yellow color

(-) Red

35
Q

DNase (+)
CUT (-)
Hockey puck colony

A

Moraxella catarrhalis

MTAP 1 Bacte (93 decks)

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