(Part 2) 2.5.2 Coryne Media and Test Flashcards
A filter paper strip impregnated with diphtheria antitoxin is laid along the center of a special agar plate on a line at right angles to the inoculum lines
Elek Test
What is the positive result of Elek Test?
White Precipitin line at 45 degrees angle
5% SBA and CHOC can help grow Coryne?
T or F
T
What is the enrichment media use for Coryne?
Loeffler’s medium
What’s the ingredients of Loeffler’s medium that helps the growth of Coryne and the production of metachromatic granules?
Serum and Egg
What are the Selective and Differential agar for Coryne
Cystine tellurite blood agad
Modified Tinsdale agar
Incubation period for selective-differential of Coryne?
48 hrs
Inhibitor for Selective - differential agar for coryne?
Potassium tellurite
What is the colony characteristics of Coryne in Cystine telluride agar
Black or Brown w/ Brown halo
What’s the reason for brown halo?
Due to cystinase enzyme activity which hydrolyzes Cystine
Which among the Coryne spp. causes Brown halo on tinsdale?
C. diptheriae
C. ulcerans
C. pseudotuberculosis
Which among the Coryne are lipophilic in Tween 80
C. jeikeium
C. urealyticum
What are the Reagent of nitrate?
Sulfanilic acid
Alpha naphthylamine
What is the agar for Urease
Christensen urea agar
End product of nitrate test?
Nitrite
