(Part 2) 2.10.3 Haemophilus Specimen processing and microsopic morphology Flashcards
Common specimen for Haemophilus?
Sterile specimens:
Blood CSF
Mid ear exudate
Synovial fluid
Up and low respiratory tract
Swabs from conjunctivae
Vaginal swabs
Abscess drainage
How to increase the sensitivity of Haemophilus for gram stain esp. CSF?
Centrifuged 2000rpm for 10 mins then smear the pellet to slide
Incubation of CHOC agar?
35-37 with 5-10% CO2
What is the selective medium that may be used for the isolation of H influenza from the respiratory secretion of patients with cystic fibrosis
Horse blood-bacitracin agar
Microscopic for H.ducreyi shows gram neg coccobacili which is commonly referred to as
Schools of fish / Railroad tracks / Finger prints
For stripp test, what media should be used?
Transparent media such as MHA and TSA
What does it mean if Haemophilus grows around X strip?
Requires X factor
What does it mean if Haemophilus grows around V strip
Requires V factor
What does it mean if Haemophilus grows around both strip
Requires both X and V factor
Haemophilus requiring X and V factor?
H. influenzae
H. aegypticus
H. haemolyticus
Haemophilus requiring V factor?
H. parainfluenzae
H. parahaemolyticus
H. paraphrohaemolyticus
Haemophilus requiring X factor?
H. ducreyi
H. ducreyi MOT?
Sexually transmitted
This used to determine X and V factor req as well as hemolysis on BAP
Haemophilus Quad plate
What type of BAP is used in certain part of Haemophilus Quad plate?
Horse Blood agar
