(Part 2) 2.23.1 Mycobacterium Flashcards
Atm req for Mycobacterium?
O. aerobic
Mycobacterium, motile
T or F
F
Mycobacterium is Gram positive
T or F
T
Spp. of Mycobacterium that caust the most cases of human tuberculosis
M. tuberculosis
Primary reservoir for tuberculosis?
a. Cavitary disease
b. Pulmonary disease
c. Kidney disease
d. GI disease
a
MOT of M. tuberculosis
Inhalation of droplet nuclei
Skin test to identify individuals infected with M. tuberculosis
a. PPD
b. T-spot TB test
c. Quantiferon-TB gold
PPD (Purified protein derivative)
In PPD, After 48-72 hours, infected individuals show erythema and
induration (+)
T or F
T
In PPD (+) is <10
t or F
F
> 10
An assay which measures T cell that have been activated by M. tuberculosis antigens
a. PPD
b. T-spot TB test
c. Quantiferon-TB gold
b
Based on the quantification of Interferon-Gamma released from sensitized lymphocytes in heparinized whole blood that has been incubated overnight
a. PPD
b. T-spot TB test
c. Quantiferon-TB gold
c
Sample used in Quantiferon-TB Gold
a. Serum
b. Whole blood without Plasma
c. Heparinized whole Blood
d. Coagulated Whole Blood
c
in Nontuberculous Mycobacteria (NTM) , membaers are opportunistic pathogens and not usually transmitted from person to person
T or F
T
Slow growing NTM, pigmented colonies in both light and Dark
a. Photochromogens
b. Scotochromogens
c. Non-Photochromogens
d. Rapid growers
b
Slow growing NTM, pigmented colonies only in light
a. Photochromogens
b. Scotochromogens
c. Non-Photochromogens
d. Rapid growers
a
Slow growing NTM, Non-pigmented colonies in regardless if light or Dark
a. Photochromogens
b. Scotochromogens
c. Non-Photochromogens
d. Rapid growers
c
NTM colonies grow in less than 7 days or earlier
a. Photochromogens
b. Scotochromogens
c. Non-Photochromogens
d. Rapid growers
d
M. kansasii
M. asiaticum
M. marinum
M. intermedium
M. branderi
(KAMIB, Only “K”, “M”, “B” in the NTM spp. and in this Categories)
a. Photochromogens
b. Scotochromogens
c. Non-Photochromogens
d. Rapid growers
a
may spp sa ibang categories na nag sisimula sa “A” and “I”
Mycobacterium terrae complex
Mycobacterium avium complex
M. gastri
(TAG, Only “T” is unique in this category)
a. Photochromogens
b. Scotochromogens
c. Non-Photochromogens
d. Rapid growers
c
M. szulgai
M. scrofulaceum
M. gordonae
M. europeaeum
M. interjectum
(GoSS In Europe, Only “S” and “E” are unique in this categry)
a. Photochromogens
b. Scotochromogens
c. Non-Photochromogens
d. Rapid growers
b
M. abscessus subsp. abscessus
M. chelonae,
M. fortuitum
(FAC, Only “F” and “C” is unique)
a. Photochromogens
b. Scotochromogens
c. Non-Photochromogens
d. Rapid growers
d
The only Mycobacterium that is noncultivable
a. M. tuberculosis
b. M. kansasii
c. M. leprae
d. M. terrae complex
c
Mycobacterium Leprae causes?
a. Hansen disease
b. Gas gangrene
c. Manuel disease
d. Ginger disease
a
MOT of M. leprae
Inhalation of nasal secretions
M. leprae cannot be cultivated in vitro
T or F
T
M. leprae can be cultivated in the armadillo and footpads of mice
T or F
T
PCR assays have been used to detect and identify M. leprae in infected tissues
T or F
T
Most specimen for Mycobacterium are respiratory samples
T or F
T
Sputum collection guidelines recommend collection of ______ specimens at least _______ apart
three, 8 hours
Decontamination is necessary for non-sterile specimens to kill bacterial contaminants, Example of Decontaminant?
a. Ethanol
b. Sodium chloride
c. Ethyl acid
d. Sodium hydroxide
d
Digestion is necessary for sputum which involves splitting the disulfide bonds that may trap mycobacteria, example of digesting agent?
a. EDTA
b. NALC
c. YMMS
d. PMRA
b. NALC (N-acetyl-L-cysteine)
After digestion and decontamination, specimens are concentrated by centrifugation at _______
a. 2000 x g
b. 1000 x g
c. 4000 x g
d. 3000 x g
d
