(Part 1) 1.14 Acid Fast Stain

Question 1

Name of for hot method?

Answer 1

Ziehl Neelson

Question 2

Name for Cold method?

Answer 2

Kinyoun

Question 3

What is the primary stain for hot and cold method?

Answer 3

Carbolfuchsin

Question 4

What is the mordant for Hot method?

Answer 4

Steam

Question 5

What is the mordant for cold method?

Answer 5

Tergitol

Question 6

What is the decolorizer for acid fast stain?

Answer 6

Acid alcohol

3% HCL
95% Ethanol

Question 7

What is the secondary stain for hot and cold method?

Answer 7

Methylene blue

Question 8

What is the result for Hot and Cold method?

Answer 8

AF: Pink
NonAF: Blue

Question 9

What is the name for the fluorescence stain?

Answer 9

Auramine Rhodamine

Question 10

Other name for Auramine Rhodamine?

Answer 10

Truant method

Question 11

PRimary stain for truant method?

Answer 11

Auramine Rhodamine fluorescence stain

Question 12

Mordant for Truant stain?

Answer 12

None

Question 13

Secondary stain for Truant method

Answer 13

Potassium permanganate

Question 14

What happens in potassium permanganate?

Answer 14

Darkens the BG

Question 15

Result for Auramine Rhodamine

Answer 15

Yellow - Orange Fluorescence