(Part 2) 2.20.3 Enterobacteriaceae Biochemical test and Media [part1]

Question 1

All enterobacteriaceae are motile except?

(Base on Handouts)

Answer 1

Shigella
Klebsiella
Yersinia

SKY

Question 2

Wat is the Colony appearance of E. coli in MAC?

Answer 2

Pink colonies

Question 3

What is the colony appearance of E.coli in EMB?

Answer 3

Pink/Purple colonies with Green metallic sheen

Question 4

E. coli is Positive for Acetate utilization

T or F

Answer 4

T

Question 5

All diarrheal E.coli are positive in Sorbitol Except?

Answer 5

EHEC (E.coli O157:H7)

Question 6

MUG test for EHEC if Forms Fluorescent

T or F

Answer 6

F

No fluorescent

Question 7

Colony appearance of Klebsiella in MAC?

Answer 7

Pink colonies

Question 8

Colony appearance of K. pneumoniae in EMB?

Answer 8

Pink mucoid colonies

Question 9

Serratia is DNAse negative

T or F

Answer 9

F

Question 10

Proteus is a rapid urea producet

T or F

Answer 10

T

Question 11

Which among the Proteus is indole positive?

Answer 11

P. vulgaris

Question 12

Which among the proteus is Ornithine positive?

Answer 12

P. mirabilis

Question 13

Edwardsiella is H2S producer and indole Positive

T or F

Answer 13

T

Question 14

Which among Citrobacter and Salmonella is positive in Urease

Answer 14

Citrobacter

Question 15

Which among Citrobacter and Salmonella is positive in LDC

Answer 15

Salmonella

Question 16

Salmonella is H2S producer

T or F

Answer 16

T

Question 17

Salmonella is negative for Phenylalanine deaminase test and urease test

T or F

Answer 17

T

Question 18

Shigella biochemical test are all negative including LOA except for VP

T or F

Answer 18

F

MR

Question 19

Shigella spp. with serotype A

Answer 19

S. dysenteriae

Question 20

Shigella spp. with serotype B

Answer 20

S. flexneri

Question 21

Shigella spp. with serotype C

Answer 21

S. boydii

Question 22

Shigella spp. with serotype D

Answer 22

S. sonnei

Question 23

All Shigella ferments mannitol except

Answer 23

S. dysentariae

Question 24

The only Shigella that has Ornithine DC

Answer 24

S. sonnei

Question 25

Mannitol (-)
Ornithine (-)
Serotype (a)

Answer 25

S. dysenteriae

Question 26

Mannitol (+)
Ornithine (-)
Serotype (c)

Answer 26

S. boydii

Question 27

Mannitol (+)
Ornithine (-)
Serotype (b)

Answer 27

S. flexnerii

Question 28

Mannitol (+)
Ornithine (+)
Serotype (d)

Answer 28

S. sonnei

Question 29

Colonies of Yersinia pestis in SBA are ________ at 24 hrs and __________ at 48 hrs

Answer 29

Pinpoint, Cauliflower

Question 30

Broth culture of Y. pestis exhibit what pattern?

Answer 30

Stalactite pattern

Question 31

What media can we observe Bull’s-eye colonies of a certain yersinia?

Answer 31

Cefsulodin-Irgasan novobiocin agar

Question 32

What Yersinia causes Bull’s eye colonies in CIN

Answer 32

Y. enterocolitica

Question 33

What are the Rapid Lactose fermenter?

Answer 33

EEK

E. coli
Enterobacter aerogenes
Enterobacter cloacae
K. pneumoniae
K. oxytoca

Question 34

What are the Late lactose fermenter?

Answer 34

Serratia
Citrobacter

Question 35

Example of Non lactose fermenter

Answer 35

PPM
Yersinia
Edwardsiella
Salmonella
Shigella
Hafinia

PPM YESSH

Question 36

What are/is the enzyme/s can be seen in RLF?

Answer 36

Lactose permease
Beta galactosidase

Question 37

What are/is the enzyme/s seen in LLF

Answer 37

Beta galactosidase

Question 38

What are the enzyme seen in NLF?

Answer 38

None

Question 39

ONPG is commonly used to detect?

Answer 39

LLF

Question 40

Meaning of ONPG?

Answer 40

Othro-Nitrophenyl-B-D-galactopyranoside

Question 41

Positive indicator of ONPG?

Answer 41

Yellow