(Part 2) 2.20.3 Enterobacteriaceae Biochemical test and Media [part1] Flashcards
All enterobacteriaceae are motile except?
(Base on Handouts)
Shigella
Klebsiella
Yersinia
SKY
Wat is the Colony appearance of E. coli in MAC?
Pink colonies
What is the colony appearance of E.coli in EMB?
Pink/Purple colonies with Green metallic sheen
E. coli is Positive for Acetate utilization
T or F
T
All diarrheal E.coli are positive in Sorbitol Except?
EHEC (E.coli O157:H7)
MUG test for EHEC if Forms Fluorescent
T or F
F
No fluorescent
Colony appearance of Klebsiella in MAC?
Pink colonies
Colony appearance of K. pneumoniae in EMB?
Pink mucoid colonies
Serratia is DNAse negative
T or F
F
Proteus is a rapid urea producet
T or F
T
Which among the Proteus is indole positive?
P. vulgaris
Which among the proteus is Ornithine positive?
P. mirabilis
Edwardsiella is H2S producer and indole Positive
T or F
T
Which among Citrobacter and Salmonella is positive in Urease
Citrobacter
Which among Citrobacter and Salmonella is positive in LDC
Salmonella
Salmonella is H2S producer
T or F
T
Salmonella is negative for Phenylalanine deaminase test and urease test
T or F
T
Shigella biochemical test are all negative including LOA except for VP
T or F
F
MR
Shigella spp. with serotype A
S. dysenteriae
Shigella spp. with serotype B
S. flexneri
Shigella spp. with serotype C
S. boydii
Shigella spp. with serotype D
S. sonnei
All Shigella ferments mannitol except
S. dysentariae
The only Shigella that has Ornithine DC
S. sonnei
Mannitol (-)
Ornithine (-)
Serotype (a)
S. dysenteriae
Mannitol (+)
Ornithine (-)
Serotype (c)
S. boydii
Mannitol (+)
Ornithine (-)
Serotype (b)
S. flexnerii
Mannitol (+)
Ornithine (+)
Serotype (d)
S. sonnei
Colonies of Yersinia pestis in SBA are ________ at 24 hrs and __________ at 48 hrs
Pinpoint, Cauliflower
Broth culture of Y. pestis exhibit what pattern?
Stalactite pattern
What media can we observe Bull’s-eye colonies of a certain yersinia?
Cefsulodin-Irgasan novobiocin agar
What Yersinia causes Bull’s eye colonies in CIN
Y. enterocolitica
What are the Rapid Lactose fermenter?
EEK
E. coli
Enterobacter aerogenes
Enterobacter cloacae
K. pneumoniae
K. oxytoca
What are the Late lactose fermenter?
Serratia
Citrobacter
Example of Non lactose fermenter
PPM
Yersinia
Edwardsiella
Salmonella
Shigella
Hafinia
PPM YESSH
What are/is the enzyme/s can be seen in RLF?
Lactose permease
Beta galactosidase
What are/is the enzyme/s seen in LLF
Beta galactosidase
What are the enzyme seen in NLF?
None
ONPG is commonly used to detect?
LLF
Meaning of ONPG?
Othro-Nitrophenyl-B-D-galactopyranoside
Positive indicator of ONPG?
Yellow
