(Part 2) 2.12.2 Brucella media, and tests

Question 1

Brucella:

Catalase (-)
Oxidase (+)
Urease (-)

Which of the following is wrong?

Answer 1

Catalase and Urease (All of them are positive)

Question 2

This is frequently used to diagnose Brucellosis

Answer 2

Serologic test (Serum agglutination test)

Question 3

What is the dilution titer for serologic test that is considered to be diagnostic for Brucellosis?

Answer 3

1:160

Question 4

Brucella agar with __________ can be used as a media for Brucella growth

Answer 4

5% horse/rabbit serum

Question 5

Incubation period of brucella agar with 5% horse/rabbit serum?

Answer 5

3 weeks in 5-10% CO2

Question 6

Media for Brucella that has a biphasic system containing both agar and broth

Answer 6

Castañeda bottle

Question 7

Which of the following are rapid urease producer (15 mins)?

B. canis
B. suis
B. melitensis
B. abortus

Answer 7

B. canis
B. suis

Question 8

Which of the following is the only H2S producer?

B. canis
B. suis
B. melitensis
B. abortus

Answer 8

B. abortus

Question 9

How many hrs for B. abortus and B. melitensis to produce urease?

Answer 9

2hrs

Question 10

Which of the following is/are inhibited by thionin?

B. canis
B. suis
B. melitensis
B. abortus

Answer 10

B. abortus

Question 11

Which of the following is/are inhibited by basic fuchsin?

B. canis
B. suis
B. melitensis
B. abortus

Answer 11

B. canis
B. suis

Question 12

What animal can B. abortus infect?

Answer 12

Cattle

Question 13

What animal can B. suis infect?

Answer 13

Pigs

Question 14

What animal can B. melitensis infect?

Answer 14

Sheep and goats

Question 15

What animal can B. canis infect?

Answer 15

Dog

Question 16

Urease (15 mins)
H2S (-)
Thionin (+)
Basic fuchsin (-)

Patient was bitten by German shepard

Answer 16

B. canis

Question 17

Urease (15 mins)
H2S (-)
Thionin (+)
Basic fuchsin (-)

Patient was working as a butcher

Answer 17

B. suis

Question 18

Urease (2 hrs)
H2S (+)
Thionin (-)
Basic fuchsin (+)

Answer 18

B. abortus

Question 19

Urease (2 hrs)
H2S (-)
Thionin (-)
Basic fuchsin (+)

Answer 19

B. melitensis

Question 20

What dye can inhibit B. abortus

Answer 20

Thionin

Question 21

What dye can inhibit B. canis and B. suis

Answer 21

Basic fuchsin

Question 22

What specimen for Brucella is more sensitive than blood?

Answer 22

Bone marrow