(Part 2) 2.4.2 Media and Test for Bacillus

Question 1

This test is used for the growth and enhancement of endospores from clinical specimens

Answer 1

Heat Shock treatment

Question 2

Temp and duration for heat shock treatment?

Answer 2

70C for 30 mins

or

80C for 10 mins

Question 3

Principle of Heat shock treatment?

Answer 3

Bacillus is heated which causes the vegetative cell to be killed due to unfavorable environment, turning it into spore. After heat treatment, the spores are plated to culture medium along with a sample of untreated specimen

Question 4

5% SBA, CHOC, and PEA can be use to grow Bacillus

T or F

Answer 4

T

Question 5

What media is used for isolation from contaminated specimens?

Answer 5

Polymyxin-Lysozyme-EDTA-thallous acetate

(PLET)

Question 6

This media is used to induce B. anthracis capsule formation

Answer 6

Bicarbonate agar

Question 7

What is the colony characteristics of B. anthracis

Answer 7

Irregular swirling projection “Medusa Head or Comet Tail”

Question 8

What is the colony characteristic for B. cereus

Answer 8

Large and feathery

Question 9

Which among the Bacillus is Beta hemolytic?

Answer 9

B. cereus

Question 10

Which among the bacillus is motile?

Answer 10

B. cereus

Question 11

Which among the bacillus are positive for lecithinase and catalase?

Answer 11

Both

Question 12

What is the media for Lecithinase test?

Answer 12

Egg yolk agar

Question 13

What is the positive indicator for Lecithinase test?

Answer 13

Opaque