P2 BIOCHEMISTRY

Question 1

heme protein found in cardiac & red skeletal muscles

Answer 1

Myoglobin

Question 2

Functions of myoglobin

Answer 2

• reservoir for O2
• O2 carrier

Question 3

type of amino acids present inside myoglobin

Answer 3

Hydrophobic

Question 4

type of amino acids present on the surface of myoglobin

Answer 4

hydrophilic

Question 5

the proximal histidine number

Answer 5

His 93

Question 6

the distal histidine number

Answer 6

His 64

Question 7

His 93 function

Answer 7

forming bound with heme iron

Question 8

His 64 function

Answer 8

Stabilising the bound to O2

Question 9

main function of haemoglobin

Answer 9

Transport of O2

Question 10

low oxygen affinity form of Hb is known as

Answer 10

deoxy form / T conformation / tense form

Question 11

high oxygen affinity form of Hb is known as

Answer 11

oxy form / R confirmation / relaxed form

Question 12

the curve shown below is known as

Answer 12

Oxygen dissociation curve

Question 13

the normal shape of the curve for haemoglobin

Answer 13

sigmoidal in shape

Question 14

the normal shape of the curve for myoglobin

Answer 14

Hyperbolic

Question 15

partial pressure at which Hb is 50% saturated with O2

Answer 15

P50

Question 16

Incase of disease , Hb O2 affinity curve shift to

Answer 16

the curve shifts to right or left

Question 17

incase of increased P50 , the curve will shift to

Answer 17

right

Question 18

incase of decreased P50 , the curve will shift to

Answer 18

left

Question 19

incase of increased BPG , the curve will shift to

Answer 19

RIGHT

Question 20

incase of decreased BPG , the curve will shift to

Answer 20

left

Question 21

type of Hb that has a high affinity for O2

Answer 21

HbF

Question 22

Substitution of which AA causes sickle cell disease

Answer 22

from glutamic acid to valine acid

Question 23

Def. in beta chain production with increase in alpha chain

Answer 23

beta thalassemia

Question 24

Def. in alpha chain production with increase in beta chain

Answer 24

alpha thalassemia

Question 25

when all of the 4 alpha globin genes are affected it is known as

Answer 25

hydrops fetalis

Question 26

incase of 2 affected alpha globin the Pt will have

Answer 26

alpha thalassemia trait

Question 27

incase of 3 alpha globin genes affected Pt will have

Answer 27

HbH disease

Question 28

name of the SHUNT that is interrupted incase of G6PD def.

Answer 28

hexose monophosphate shunt

Question 29

main function of NADPH released in pentose phosphate pathway

Answer 29

reduction of glutathione molecule

Question 30

Enzyme that is used to reduce glutathione molecule

Answer 30

Glutathione reductase

Question 31

Genetic predisposition of G6PD def.

Answer 31

X-linked recessive

Question 32

protein that is aggregated and accumulated in G6PD def. seen in blood smear as

Answer 32

heinz bodies

Question 33

clinical manifestations of G6PD def.

Answer 33

• acute hemolytic anemia
• neonatal jaundice

Question 34

these cases can cause haemolytic anemia in G6PD def.

Answer 34

• oxidant drugs
• favism

Question 35

G6PD carriers are protected against

Answer 35

Malaria

Question 36

severe hemolysis dut to G6PD def is manifested as

Answer 36

methemoglobinemia