molecular techniques Flashcards by Sarah Heath | Brainscape

Q

PCR

A

Polymerase Chain Reaction. Amplify DNA via cycle after cycle of replication.

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

PCR steps

A

Denaturation by heat ( >90C), add a bunch of primers and Anneal, elongation (Taq polymerase, 70C), Repeat

How well did you know this?

1

Not at all

2

3

4

5

Perfectly

Q

What is an endonuclease?

A

Restriction Enzyme. sticky ends

How well did you know this?

1

Not at all

2

3

4

5

Perfectly