MLSP: Microbiology Flashcards
Allow room air to pass into
cabinet and around the area and
material within, sterilizing only the
air to be exhausted
CLASS I
Consist of negative pressure and operated in open front
CLASS I
Air flows in “sheets”, which serves
as a barrier to particle from outside
the cabinet
CLASS II CLASS II
Direct the flow of contaminated
air into the filters, also called as VERTICAL LAMINAR FLOW BSCs
CLASS II
Self-contained, and 70% of the air
is recirculated [most common in
clinical microbiology section]
CLASS IIA
Selected for radioisotopes, toxic
chemicals or carcinogenic samples
CLASS IIB
Prokaryotic unicellular organisms that lack a true nucleus and nuclear membrane
BACTERIA
Single, closed, circular chromosomes of double-
stranded DNA called?
NUCLEOID
small circular molecules o
extrachromosomal circular DNA
PLASMIDS
May either be GRAM POSITIVE [violet] or GRAM
NEGATIVE [pink or reddish pink]
BACTERIA
asexual reproduction of bacteria
BINARY FISSION
range from 0.2 to 2um in diameter
and 1 to 6um in length
Bacteria size
Principal stain used for microscopic examination of bacteria
GRAM STAIN
T or F
Gram stain is first devised by Hans Christian Gram during the late 19th century
True
take up the basic dye, crystal violet
Gram-positive
allow crystal violet dye to wash out easily with decolorizer alcohol or acetone
Gram-negative
CELL WALL
Thick (peptidoglycan)
Gram positive
CELL WALL
Thin (peptidoglycan)
Gram negative
LIPOPOLYSACCHARIDE
Absence
Gram positive
LIPOPOLYSACCHARIDE
Presence
Gram negative
GRAM STAINING
Purple
Gram positive
GRAM STAINING
Red or Pink
Gram negative
REPRESENTATION
Purple or Violet result
Gram positive
REPRESENTATION
Pink result
Gram negative
spherical-shaped cells
COCCI
rod-shaped cells
BACILLI
spiral shaped cells
SPIRILLA
comma-shaped cells
VIBRIOS
T or F
FOUR BASIC MORPHOLOGICAL TYPES OF BACTERIA; COCCI, BACILLI, SPIRILLA, VIBRIOS
True
Process by which bacteria catabolize carbohydrates to produce energy
FERMENTATION
Also known as oxidation. Process of bacterial energy generation rather than fermentation
RESPIRATION
Tongue should be depressed before swabbing
between the tonsillar pillars and behind the uvula
THROAT (SWABS)
The cheek, tongue, and teeth should NOT be
touched
THROAT (SWABS)
Expectorated specimens from deep cough should
be collected into a sterile specimen cup
SPUTUM
Should never be taken from the toilet and should not be contaminated with urine
STOOL
Midstream clean-catch is the most common
collection method
URINE
Culture for catheterized urine specimens usually have less contaminating bacterial flora
URINE
2-3 cultures should be collected at random times during 24-hour period
BLOOD
20-30mL of blood per culture is collected
ADULT
1-5mL of blood per culture is collected
INFANTS
Should be collected aseptically by PHYSICIAN
CEREBROSPINAL FLUID
Should be processed immediately and not exposed to heat or refrigeration
CEREBROSPINAL FLUID
Exudates may be expressed from the urethral
orifice or a small-diameter swab inserted 3-4
cm into the urethra
MEN (penile discharge)
A sterile swab is inserted into the cervix with an
aid of speculum, swab is rotated and allowed to remain for a few seconds
WOMEN (vaginal discharge)
Anaerobic specimens must be transported in an anaerobic system
HANDLING AND TRANSPORT
T or F
Aerobic set-up is okay to be exposed in oxygen
True
T or F
Anaerobic should not be exposed to oxygen
True
T or F
Swab samples are not allowed to dry out
True
T or F
Most specimen can be held at 2°C to 8°C
True
Support the growth of a wide range of
microorganisms and are considered non
selective because, the growth of most
organisms is supported
Example: Blood Agar Plate and Chocolate Agar
Nutritive Media
Microorganisms that can be distinguished on
the basis if certain growth characteristics
evident on the medium
Example: Blood Agar Plate
Differential Media
Support the growth of one group of organisms,
but not another, by adding antimicrobials, dyes,
or alcohol to a particular medium
Example: MacConkey Agar [only allow gram-
negative organisms], Columbia agar with
colistin and nalidixic acid (CAN) Agar
Selective Media
Also called supplemental or enrichment broth
For detection of small numbers of organisms
present
Example: Thioglycollate (Thio) broth, brain-
heart infusion broth (BHIB) and tryptic soy
broth (TSB)
Backup Broth
Used when specific organisms are suspected
Example: Mannitol salt agar, bismuth sulfite
agar, Campylobacter agar, Thiosulfate-citrate-
bile salts-sucrose (TCBS) agar
Specialized Media
INCUBATION
35°C-37°C
Normal incubation temperature
INCUBATION
42°C to 45°C
for stool cultures detection of
Campylobacter jejuni
T or F
Anaerobic jars, bags, or an anaerobic chamber are appropriate for incubation of anaerobic cultures
True
