MLSP: Microbiology

Question 1

Allow room air to pass into
cabinet and around the area and
material within, sterilizing only the
air to be exhausted

Answer 1

CLASS I

Question 2

Consist of negative pressure and operated in open front

Answer 2

CLASS I

Question 3

Air flows in “sheets”, which serves
as a barrier to particle from outside
the cabinet

Answer 3

CLASS II CLASS II

Question 4

Direct the flow of contaminated
air into the filters, also called as VERTICAL LAMINAR FLOW BSCs

Answer 4

CLASS II

Question 5

Self-contained, and 70% of the air
is recirculated [most common in
clinical microbiology section]

Answer 5

CLASS IIA

Question 6

Selected for radioisotopes, toxic
chemicals or carcinogenic samples

Answer 6

CLASS IIB

Question 7

Prokaryotic unicellular organisms that lack a true nucleus and nuclear membrane

Answer 7

BACTERIA

Question 8

Single, closed, circular chromosomes of double-
stranded DNA called?

Answer 8

NUCLEOID

Question 9

small circular molecules o
extrachromosomal circular DNA

Answer 9

PLASMIDS

Question 10

May either be GRAM POSITIVE [violet] or GRAM
NEGATIVE [pink or reddish pink]

Answer 10

BACTERIA

Question 11

asexual reproduction of bacteria

Answer 11

BINARY FISSION

Question 12

range from 0.2 to 2um in diameter
and 1 to 6um in length

Answer 12

Bacteria size

Question 13

Principal stain used for microscopic examination of bacteria

Answer 13

GRAM STAIN

Question 14

T or F
Gram stain is first devised by Hans Christian Gram during the late 19th century

Answer 14

True

Question 15

take up the basic dye, crystal violet

Answer 15

Gram-positive

Question 16

allow crystal violet dye to wash out easily with decolorizer alcohol or acetone

Answer 16

Gram-negative

Question 17

CELL WALL
Thick (peptidoglycan)

Answer 17

Gram positive

Question 18

CELL WALL
Thin (peptidoglycan)

Answer 18

Gram negative

Question 19

LIPOPOLYSACCHARIDE
Absence

Answer 19

Gram positive

Question 20

LIPOPOLYSACCHARIDE
Presence

Answer 20

Gram negative

Question 21

GRAM STAINING
Purple

Answer 21

Gram positive

Question 22

GRAM STAINING
Red or Pink

Answer 22

Gram negative

Question 23

REPRESENTATION
Purple or Violet result

Answer 23

Gram positive

Question 24

REPRESENTATION
Pink result

Answer 24

Gram negative

Question 25

spherical-shaped cells

Answer 25

COCCI

Question 26

rod-shaped cells

Answer 26

BACILLI

Question 27

spiral shaped cells

Answer 27

SPIRILLA

Question 28

comma-shaped cells

Answer 28

VIBRIOS

Question 29

T or F FOUR BASIC MORPHOLOGICAL TYPES OF BACTERIA; COCCI, BACILLI, SPIRILLA, VIBRIOS

Answer 29

True

Question 30

Process by which bacteria catabolize carbohydrates to produce energy

Answer 30

FERMENTATION

Question 31

Also known as oxidation. Process of bacterial energy generation rather than fermentation

Answer 31

RESPIRATION

Question 32

Tongue should be depressed before swabbing between the tonsillar pillars and behind the uvula

Answer 32

THROAT (SWABS)

Question 33

The cheek, tongue, and teeth should NOT be touched

Answer 33

THROAT (SWABS)

Question 34

Expectorated specimens from deep cough should be collected into a sterile specimen cup

Answer 34

SPUTUM

Question 35

Should never be taken from the toilet and should not be contaminated with urine

Answer 35

STOOL

Question 36

Midstream clean-catch is the most common collection method

Answer 36

URINE

Question 37

Culture for catheterized urine specimens usually have less contaminating bacterial flora

Answer 37

URINE

Question 38

2-3 cultures should be collected at random times during 24-hour period

Answer 38

BLOOD

Question 39

20-30mL of blood per culture is collected

Answer 39

ADULT

Question 40

1-5mL of blood per culture is collected

Answer 40

INFANTS

Question 41

Should be collected aseptically by PHYSICIAN

Answer 41

CEREBROSPINAL FLUID

Question 42

Should be processed immediately and not exposed to heat or refrigeration

Answer 42

CEREBROSPINAL FLUID

Question 43

Exudates may be expressed from the urethral orifice or a small-diameter swab inserted 3-4 cm into the urethra

Answer 43

MEN (penile discharge)

Question 44

A sterile swab is inserted into the cervix with an aid of speculum, swab is rotated and allowed to remain for a few seconds

Answer 44

WOMEN (vaginal discharge)

Question 45

Anaerobic specimens must be transported in an anaerobic system

Answer 45

HANDLING AND TRANSPORT

Question 46

T or F Aerobic set-up is okay to be exposed in oxygen

Answer 46

True

Question 47

T or F Anaerobic should not be exposed to oxygen

Answer 47

True

Question 48

T or F Swab samples are not allowed to dry out

Answer 48

True

Question 49

T or F Most specimen can be held at 2°C to 8°C

Answer 49

True

Question 50

Support the growth of a wide range of microorganisms and are considered non selective because, the growth of most organisms is supported Example: Blood Agar Plate and Chocolate Agar

Answer 50

Nutritive Media

Question 51

Microorganisms that can be distinguished on the basis if certain growth characteristics evident on the medium Example: Blood Agar Plate

Answer 51

Differential Media

Question 52

Support the growth of one group of organisms, but not another, by adding antimicrobials, dyes, or alcohol to a particular medium Example: MacConkey Agar [only allow gram- negative organisms], Columbia agar with colistin and nalidixic acid (CAN) Agar

Answer 52

Selective Media

Question 53

Also called supplemental or enrichment broth For detection of small numbers of organisms present Example: Thioglycollate (Thio) broth, brain- heart infusion broth (BHIB) and tryptic soy broth (TSB)

Answer 53

Backup Broth

Question 54

Used when specific organisms are suspected Example: Mannitol salt agar, bismuth sulfite agar, Campylobacter agar, Thiosulfate-citrate- bile salts-sucrose (TCBS) agar

Answer 54

Specialized Media

Question 55

INCUBATION 35°C-37°C

Answer 55

Normal incubation temperature

Question 56

INCUBATION 42°C to 45°C

Answer 56

for stool cultures detection of Campylobacter jejuni

Question 57

T or F Anaerobic jars, bags, or an anaerobic chamber are appropriate for incubation of anaerobic cultures

Answer 57

True