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MIDTERMS & FINALS > CHEM: CHROMA > Flashcards

CHEM: CHROMA Flashcards

1
Q

• Is a technique used to separate and identify the
components of a mixture.

A

CHROMATOGRAPHY

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2
Q

• Works by allowing the molecules present in the
mixture to distribute themselves between a
stationary and a mobile medium.
• Molecules that spend most of their time in the
mobile phase are carried along faster.

A

CHROMATOGRAPHY

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3
Q

is a laboratory technique that
separates components within a mixture by using
the differential affinities of the components for a
mobile medium and for a stationary adsorbing
medium through which they pass

A

CHROMATOGRAPHY

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4
Q

showing a difference, distinctive

A

Differential

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5
Q

natural attraction or force between things

A

Affinity

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6
Q

gas or liquid that carries the components (mobile phase)

A

Mobile Medium

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7
Q

the part of the apparatus that does not move with the sample (stationary phase)

A

Stationary Medium

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8
Q

examine a mixture, its components, and their relations to one another

A

Analyze

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9
Q

determine the identity of a mixture or components based on known components

A

Identify

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10
Q

separate components in order to isolate one of interest for further study

A

purify

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11
Q

determine the amount of the a mixture and/or the components present in the sample

A

Quantify

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12
Q

can also be used to separate the colored pigments in plants

A

Chromatography

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13
Q

(t or f)

We can determine the RF (or retardation factor) by
measuring the distance the pigments traveled up
the strip and the distance the water traveled

A

true

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14
Q

For substances that are very soluble in the liquid Rf
will be close to

A

1

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15
Q

For substances that are rather insoluble in the liquid
Rf will be close to

A

0

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16
Q

• Separates dried liquid samples with a liquid solvent
(mobile phase) and a paper strip (stationary phase)

A

PAPER CHROMATOGRAPHY

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17
Q

the movement of liquid within the spaces of a porous material due to the forces of adhesion, cohesion, and surface tension.

A

Capillary Action

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18
Q

the degree to which a material (solute)
dissolves into a solvent. Solutes dissolve into
solvents that have similar properties.

A

Solubility

19
Q

(t of f)

Separation of components depends on both their
solubility in the mobile phase and their differential
affinity to the mobile phase and the stationary
phase.

A

true

20
Q

uses an absorbent material on flat glass or plastic
plates.

A

THIN LAYER CHROMATOGRAPHY

21
Q

It is used to detect pesticide or insecticide residues
in food.

A

THIN LAYER CHROMATOGRAPHY

22
Q

is also used in forensics to analyze the dye composition of fibers.

A

THIN LAYER CHROMATOGRAPHY

23
Q

(t or f)

Most samples are not colored and need to be visualized with a UV lamp.

A

true

24
Q

is used in airports to detect bombs and is used in
forensics in many different ways.

A

GAS CHROMATOGRAPHY

25
Q

It is used to analyze fibers on a persons body and
also analyze blood found at a crime scene.

A

GAS CHROMATOGRAPH

26
Q

is used to move a gaseous mixture through a column of absorbent material

A

Helium

27
Q

, called the Mobile Phase,

A

gas mixture

28
Q

usually tightly packed into a copper tube called the Column…
- which separates the gas stream into its particular components based on how “quickly” they move through the stationary phase.

A

Stationary Phase

29
Q

generates an electrical signal which is sent to a Recorder

A

Detector

30
Q

to record the signal.

A

Recorder

31
Q

A sample mixture is passed through a column
packed with solid particles which may or may not be
coated with another liquid.

A

LIQUID CHROMATOGRAPHY

32
Q

With the proper solvents, packing conditions, some
components in the sample will travel the column
more slowly than others resulting in the desired
separation.

A

LIQUID CHROMATOGRAPHY

33
Q

• is used in the world to test water samples to look
for pollution in lakes and rivers.
• is used to analyze metal ions and organic
compounds in solutions.

A

LIQUID CHROMATOGRAPHY

34
Q

uses liquids which may incorporate insoluble
molecules.

A

LIQUID CHROMATOGRAPHY

35
Q

(adsorption chromatography)
a. Normal Phase LSC
b. Reverse Phase LSC

A

Liquid/Solid Chromatography

36
Q

(partition chromatography)
a. Normal Phase LLC
b. Reverse Phase LLC

A

Liquid/Liquid Chromatography

37
Q

(exclusion chromatography)

A

Gel Permeation Chromatography

38
Q

The stationary solid surface is coated with a 2nd
liquid (the Stationary Phase) which is immiscible in
the solvent (Mobile) phase.

Partitioning of the sample between 2 phases delays
or retains some components more than others to
effect separation.

A

LIQUID-LIQUID CHROMATOGRAPHY

39
Q

separation of ____________ is based on the competition of different ionic compounds of the sample for the active sites on the ion-exchange resin (column-packing).

A

Ion-exchange Chromatography

40
Q

is a mechanical sorting of molecules based on the size of the molecules in solution.

A

Gel-Permeation Chromatography

41
Q

is a form of column chromatography used frequently in biochemistry and analytical chemistry to separate,
identify, and quantify compounds.

A

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

42
Q

utilizes a column that holds chromatographic packing
material (stationary phase), a pump that moves the
mobile phase(s) through the column, and a detector
that shows the retention times of the molecules.

A

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

43
Q

uses a polar stationary phase and a non-polar mobile phase, and works effectively for relatively polar analytes

A

Normal Phase Chromatograaphy

44
Q

has a non-polar stationary phase and an aqueous, moderately polar mobile phase

A

Reversed Phase Chromatography

MIDTERMS & FINALS (39 decks)

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