MLSP: BACTERIAL SMEAR PREP & GRAM STAINING (LAB) Flashcards
A dried preparation of bacterial cells on a glass slide
BACTERIAL SMEAR
(T or F)
- The bacteria are evenly spread out on the slide in such a concentration that they are adequately separated from one another
- The bacteria are not washed off the slide during staining
true
(t or f)
- Bacterial form is not distorted (ensure that the
material we are using is sterile and we do not kill the bacteria in the process in making the smear;mwe can kill the bacteria if the materials are heated too much or heat sterilization [to ensure that cross-contamination will not happen])
true
most common material that we’ll be using for smear preparation in bacteria
wire loop
from _____________________ there is a target circle oin the bottom of the slide
broth culture tubes
from __________________ you need two loopfuls as well and always put it into the target circle in the glass slide
plates and slants
The use of a single stain to color a bacterial cell
SIMPLE STAINING
Commonly used dyes for performing simple staining are
methylene blue,
basic fuchsin,
crystal violet
(t or f)
methylene blue, basic fuchsin, and crystal violet These are referred to as basic dyes because theynhave color-bearing ionic groups (chromophores) that are positively charged (cationic)
true
Two kinds of cells
gram positive and gram negative
colored purple
gram positive
colored red or pink
gram negative
Reagents used in gram staining:
primary stain
Crystal violet
Reagents used in gram staining:
Mordant [help enhance the primary stain]
Gram’s Iodine
Reagents used in gram staining:
decolorizer [decolorize gram positive organisms
Acetone Alcohol
