microbiology ch 4 Flashcards
Do prokaryotes have one circular chromosome?
Yes
Do prokaryotes have histones?
No
Do prokaryotes have organelles?
No
What type of cell wall do bacteria have?
Peptidoglycan cell walls
What type of cell wall to Archaea have?
Pseudomurein cell walls
How do prokaryotes divide?
By binary fission
Do eukaryotes have paired chromosomes?
Yes
Are eukaryote chromosomes in a membrane?
Yes
Do eukaryotes have histones?
Yes
Do eukaryotes have organelles?
Yes
Can eukaryotes have polysaccharide cell walls?
Yes
How do eukaryotes divide?
By mitosis
Do eukaryotes have a circular chromosome?
No
Are prokaryote chromosomes located in a membrane?
No
Do prokaryotes divide by mitosis?
No
What is the average length of bacterial cells?
2 to 8 um
What is the average diameter of bacterial cells?
0.2 to 2.0 um
Are most bacteria monomorphic?
Yes
What does monomorphic mean?
Single-shape
What does pleomorphic mean?
Many shapes
What shape is a coccus bacteria?
Round
What shape is a vibrio bacteria?
Comma shaped
What shape is a bacillus bacteria?
Pill-shaped
What shape is a spirillium bacteria?
Corkscrew shape
What shape is a spirochete bacteria?
Corkscrew shape with axial filament
What are the 5 general bacteria shapes?
Bacillus, coccus, star-shaped, rectangular, and spiral
What are the three types of spiral bacteria shapes?
Vibrio, spirillium and spirochete
What are the 5 arrangements of bacterial cells?
Pairs, clusters, chains, groups of four, and cubelike groups of eight
What shape and arrangement is Bacillus?
Bacillus (pill shape) and chain
What is the glycocalyx?
Sugar coat
How is the glycocalyx characterized?
Viscous and gelatinous
What substances is the glycocalyx made of?
Polysaccharide and/or polypeptide
What are the two types of glycocalyx?
Capsule and slime layer
Which type of glycocalyx is neatly organized and firmly attached to cell wall?
Capsule
Which type of glycocalyx is unorganized and loosely attached to the cell wall?
Slime layer
What is the sugar coat of bacteria called?
Glycocalyx
This type of glycocalyx is neatly organized
Capsule
This type of glycocalyx is loosely attached to the cell wall
Slime layer
This type of glycocalyx is unorganized
Slime layer
This type of glycocalyx is neatly organized
Capsule
Does the glycocalyx affect the virulence of a pathogen?
Yes
Do capsules prevent phagocytosis?
Yes
How does a capsule help the cell?
By forming biofilm, protecting cell, and facilitating communication
These are filamentous appendages external to the cell
Flagella
What are flagella made of?
Flagellin
What is flagellin?
A protein used to make flagella
What are the three parts of flagella?
Filament, hook, and basal body
Which part of flagella is outermost?
Filament
To what flagella part does the filament attach?
Hook
Which flagella part consists of a rod and pairs of rings?
Basal body
Which flagella part anchors flagellum to the cell and membrane?
Basal body
What movements result from flagella rotation?
Runs and tumbles
Which direction are flagella runs?
Counterclockwise
Which direction are flagella tumbles?
Clockwise
What are archaella?
Archaeal motility structures
What are archaellins made of?
Archaellin glycoproteins
These are similar structures to flagella found in archaea
Archaella
Are archaella anchored to the cell?
Yes
Are flagella anchored directly to the cell?
No
What are also known as endoflagella?
Axial filaments
What shape of bacteria are endoflagella (aka axial filaments) found in?
Spirochetes
Are endoflagella/axial filaments anchored at one end of the cell?
Yes
What kind of movement does endoflagella rotation produce?
Corkscrew motion
Spirochetes have this type of flagella
Endoflagella/axial filaments
These rotate to give cell corkscrew motion
Endoflagella/axial filaments
What protein makes up fimbriae and pili?
Pilin
What are fimbriae?
Hairlike appendages that allow for attachment
How many fimbriae can exist on a cell?
From a few to hundreds
These are hairlike appendages that allow for attachment and can number from a few to hundreds
Fimbriae
What two motility types are pili responsible for?
Gliding and twitching
How many pili can a cell have?
One or two
Are pili longer than fimbriae?
Yes
What type of pili are used in DNA transfer between cells?
Conjugation pili
These are involved in gliding and twitching motility
Pili
A cell can only have one or two of these long motility appendages
Pili
These appendages are used for DNA transfer from one cell to another
Conjugation pili
What structure prevents osmotic lysis and protects the cell membrane?
Cell wall
Can cell walls contribute to pathogenicity?
Yes
This cell structure is the site of action of some antibiotics and is used to differentiate types of bacteria
Cell wall
These prevent osmotic lysis and can contribute to pathogenicity
Cell walls
Is peptidoglycan a polymer?
Yes
What two rows repeat in peptidoglycan?
N-acetylglucosamine (NAG) and N-acetylmuramicacid (NAM)
How are peptidoglycan rows linked?
By polypeptide
What is made up of repeating rows of N-acetylglucosamine and N-acetylmuramicacid?
Peptidoglycan
What do gram-positive cell walls contain?
Thick peptidoglycan and teichoic acids
What three substances characterize teichoic acids?
Lipoteichoic acid, wall teichoic acid, and a negative charge
What do lipoteichoic acids do?
Link cell wall to plasma membrane
What do wall teichoic acids link?
Peptidoglycan
What two substances provide antigenic specificity?
Polysaccharides and teichoic acids
What three components make up the structure of gram-negative cell walls?
Thin peptidoglycan, outer membrane, and periplasmic space
What three substances does the outer membrane of gram-negative cell walls contain?
Polysaccharides, lipoproteins, and phospholipids
What does the periplasmic space contain?
Peptidoglycan
What proteins form channels through the membrane?
Porins
What is Lipid A?
An endotoxin embedded in top layer of gram-negative cell walls
Lipopolysaccharides make up which type of cell walls?
Gram-negative cell walls
This functions as an antigen in gram-negative cell walls
O polysaccharide
What do core polysaccharides do?
Provide stability
What do porins do?
Form channels through membrane
What dye forms crystals inside cells?
Crystal violet-iodine
What is the mechanism that takes place during gram-positive staining?
Alcohol dehydrates peptidoglycan and CV-I crystals do not leave
What three step mechanism takes place during gram-negative staining?
Alcohol dissolves outer membrane, leaving holes in peptidoglycan; CV-I washes out and cells are colorless; safranin added to stain cells
Alcohol dehydrates peptidoglycan in which type of staining?
Gram-positive
Safranin is added in which type of staining?
Gram-negative
How many rings are in the basal body of a gram-positive bacteria’s flagella?
2
This cell wall type produces exotoxins
Gram-positive cell wall
This cell wall type is highly susceptible to penicillin
Gram-positive cell wall
This cell wall type is disrupted by lysozymes
Gram-positive cell wall
This cell wall type has 2 rings in the basal body of its flagella
Gram-positive cell wall
What type of toxins do gram-positive cell walls produce?
Exotoxins
What type of antibiotic are gram-positive cell walls susceptible to?
Penicillin
What disrupts gram-positive cell walls?
Lysozymes
This cell wall type has 4 rings in the basal body of its flagella
Gram-negative cell wall
What toxins do gram-negative cell walls produce?
Endotoxins and exotoxins
Do gram-negative cell walls have a high susceptibility to penicillin?
No
How many rings are there in the basal body of gram-negative flagella?
4
This type of cell wall produces endotoxins and exotoxins
Gram-negative cell wall
This type of cell wall has low susceptibility to penicillin
Gram-negative cell wall
Do gram-negative cell walls only produce endotoxins?
No
Acid-fast cell walls are similar to what other type of cell wall?
Gram-positive
What substances do acid-fast cell walls have surrounding them?
Mycolic acid bound to peptidoglycan
What is mycolic acid?
A waxy lipid that binds to peptidoglycan in acid-fast cell walls
Myobacterium and Nocardia are two examples of what type of cell wall?
Acid-fast cell walls
What is used to stain acid-fast cell walls?
Carbolfuchsin
This type of cell wall is similar to gram-positive cell walls
Acid-fast cell walls
This type of cell wall has mycolic acid bound to peptidoglycan
Acid-fast cell walls
What are two examples of taxa with acid-fast cell walls?
Mycobacterium and Nocardia
This type of cell wall is stained with carbolfuchsin
Acid-fast cell walls
This is a waxy lipid that binds to peptidoglycan in acid-fast cell walls
Mycolic acid
What do mycoplasmas lack?
Cell walls
What do mycoplasmas have in their plasma membrane?
Sterols
Do Archaea typically have cell walls?
No
When Archaea do have cell walls, they are usually made of this
Pseudomurein
Archaea pseudomurein cell walls usually lack these two substances
NAM and D-amino acids
These lack cell walls
Mycoplasmas
These have sterols in their plasma membrane
Mycoplasmas
These are typically cell wall-less, or can have walls of pseudomurein
Archaea
These have cell walls lacking in NAM and D-amino acids
Archaea
What do lysozymes do to cell walls?
Hydrolyze bonds in peptidoglycan
What does penicillin do to cell walls?
Inhibits peptide bridges in peptidoglycan
This is a wall-less gram-positive cell
Protoblast
This is a wall-less gram-negative cell
Spheroplast
Are protoblasts and spheroblasts susceptible to osmotic lysis?
Yes
These are wall-less cells that swell into irregular shapes
L forms
These hydrolyze bonds in peptidoglycans
Lysozymes
This inhibits peptide bridges in peptidoglycan
Penicillin
What are protoblasts?
Wall-less gram-positive cells
What are spheroplasts?
Wall-less gram-negative cells
What are L-forms?
Wall-less cells that swell into irregular shapes
What are protoblasts and spheroplasts susceptible to?
Osmotic lysis