microbiology ch 10 Flashcards
What is taxonomy?
The science of classifying organisms
What does taxonomy show?
Degree of similarity among organisms
What is systematics/phylogeny?
The study of the evolutionary history of organisms
This is the science of classifying organisms and shows the degrees of similarity among organisms
Taxonomy
This is the study of the evolutionary history of organisms
Systematics/phylogeny
Who developed the current three domain system of phylogeny?
Carl Woese
When did Carl Woese develop the three-domain system of phylogeny that is currently used?
1978
What are the three domains of organisms?
Eukarya, Bacteria, Archaea
What developed into organelles in the development of eukaryotes?
Endosymbiotic bacteria
What did eukaryotes originate from?
Infoldings of prokaryotic plasma membranes
Endosymbiotic bacteria developed into these in eukaryotes
Organelles
These originated from infoldings of prokaryotic plasma membranes
Eukaryotes
How are organisms grouped in phylogenetic trees?
According to common properties
What do mutations accumulated in genomes serve as?
Molecular clock
What does each species from from its ancestor?
Some characteristics
These group organisms according to common properties
Phylogenetic trees
These serve as a molecular clock
Mutations accumulated in genomes
What constitutes a eukaryotic species?
A group of closely related organisms that breed among themselves
What constitutes a prokaryotic species?
A population of cells with similar characteristics
What constitutes a viral species?
A population of viruses with similar characteristics that occupies a particular ecological niche
This type of species is made up of a group of closely related organisms that breed among themselves
Eukaryotic species
This type of species is made up of a population of cells with similar characteristics
Prokaryotic species
This type of species is a population of viruses with similar characteristics that occupies a particular ecological niche
Viral species
In prokaryote classification, what is a culture?
Bacteria grown in a laboratory media
In prokaryote classification, what is a clone?
Population of cells derived from a single parent cell
In prokaryote classification, what is a strain?
Genetically different cells within a clone
This is includes bacteria grown in laboratory media
Culture
This is a population of cells derived from a single parent cell
Clone
This is made up of genetically different cells within a clone
Strain
What is the makeup of Kingdom Protista?
Catch-all kingdom for a variety of autotrophic and heterotrophic organisms
How is Kingdom Protista grouped?
Into clades based on rRNA
What are four characteristics of Kingdom Fungi?
Chemoheterotrophic; unicellular or multicellular; chitin cell walls; develop from spores/hyphal fragments
What are three characteristics of Kingdom Plantae?
Multicellular; cellulose cell walls; undergo photosynthesis
What are three characteristics of Kingdom Animalia?
Multicellular; lack cell walls; chemoheterotrophic
This kingdom is a catch-all for a variety of organisms, both autotrophic and heterotrophic, and is grouped into clades based on rRNA
Protista
Members of this kingdom are chemoheterotrophic, unicellular or multicellular, have cell walls made of chitin, and develop from spores or hyphal fragments
Fungi
Members of this kingdom are multicellular, have cellulose cell walls, and undergo photosynthesis
Plantae
Members of this kingdom are multicellular, lack cell walls, and are chemoheterotrophic
Animalia
What is organism classification?
Placing organisms in groups of related species
What is organism identification?
Matching characteristics of an unknown organism to lists of known organisms
This involves placing organisms in groups of related species
Classification
This involves matching characteristics of an unknown organism to lists of known organisms
Identification
What are morphological characteristics useful for identifying?
Eukaryotes
What is a disadvantage of using morphological characteristics to classify/identify organisms?
Morphological characteristics tell little about phylogenetic relationships
What are two types of differential staining used to identify/classify bacteria?
Gram staining and acid-fast staining
What types of bacteria is differential staining not useful for identifying/classifying?
Bacteria without cell walls
What do biochemical tests determine?
Presence of bacterial enzymes
These are useful for identifying eukaryotes, but tell little about phylogenetic relationships
Morphological characteristics
This method of identification/classification is useful for distinguishing bacteria with cell walls
Differential staining
This method of identification/classification determines the presence of bacterial enzymes
Biochemical testing
What can rapid identification methods do?
Perform several biochemical tests simulatenously
These can perform several biochemical tests simultaneously
Rapid identification methods
What is serology?
The science of serum and immune responses in serum
What do microorganisms do in serum/
Stimulate antibody formation
What can serological testing do?
Differentiate between species/strains within species
This is the study of serum and immune responses in serum
Serology
These are antigenic and stimulate the body to form antibodies in the serum
Microorganisms
This can differentiate between species/strains in serum
Serological testing
What happens in the slide agglutination test?
Bacteria agglutinate when mixed with antibodies produced in response to the bacteria
In this serological test, bacteria agglutinate when mixed with antibodies produced in response to the bacteria
Slide agglutination test
What happens in an enzyme-linked immunosorbent assay (ELISA)?
Known antibodies and an unknown type of bacterium are added to a well and a reaction identifies the bacteria
In this serological test, known antibodies and unknown bacteria are added to a well where a reaction identifies the bacteria
Enzyme-linked immunosorbent assay (ELISA)
What do fatty acid methyl esters (FAMEs) do?
Provide profiles that are constant for a particular species to identify bacteria
These provide profiles constant for a particular species and are used in bacterial identification
Fatty acid methyl esters (FAMEs)
In DNA sequencing, what do two closely related organisms have?
Similar amounts of DNA bases
GC + AT = ? in DNA base composition
100%
This method of classification/identification uses online databases to compare organisms’ similarity in DNA base composition
DNA sequencing
What does DNA fingerprinting use?
Electrophoresis of restriction enzyme digests of an organism’s DNA
In this method of classification/identification, comparing fragments from different organisms provides information on genetic similarities and differences
DNA fingerprinting
This method of classification/identification uses electrophoresis of restriction enzyme digests of an organism’s DNA
DNA fingerprinting
What does nucleic acid hybridization measure?
Ability of DNA strands from one organism to hybridize with DNA strands of another
In nucleic acid hybridization, what does a greater degree of hybridization indicate?
Greater degree of relatedness
What percentage of hybridization indicates two organisms are from the same species?
> 70%
In this method of classification/identification, the ability of DNA strands from one organism to hybridize with those of another is measured
Nucleic acid hybridization
Does a greater degree of hybridization indicated a lesser degree of relatedness in nucleic acid hybridization?
No
Does hybridization of greater than %50 indicate two organisms are the same species?
No
Does hybridization of greater than >70% indicate two organisms are the same species?
Yes
What are dichotomous keys?
Identification keys based on successive questions
What are cladograms?
Maps that show evolutionary relationships among organisms based on rRNA sequences
These are identification keys based on successive questions
Dichotomous keys
These are maps that show evolutionary relationships among organisms and are based on rRNA sequences
Cladograms