microbiology Flashcards
selective media agar type and what is it selective for?
sabouraud dextrose agar
inhibits bacteria, selective for fungi
differential media agar type and what is it for?
blood agar
differentiate streptococcus species
selective AND differential media agar type and what is it used for?
MacConkey agar
inhibits gram + selective for gram - and differentiates different gram - bacteria
psychrophilic
prefers cold
mesophilic
moderate temps PATHOGENS
thermophilic
prefers high temps
what are self-nourishing with CO2 as nutrient source?
autotrophs
what need organic/environmental carbon for nutrients?
heterotrophs
what is gram + stain?
purple
what is gram - stain?
red
what are the simple stains?
crystal violet
safranin
methylene blue
malachite green
what are acid fast stains?
red- mycobacteria
blue- other tissue
what are endospore stains?
green- endospores
red- vegetative cells
what are the gram + exceptions?
Us Um
listeria
nocardia
actinomyces
streptomyces- antiobiotic
what are gram - exceptions?
A Er
pseudomonas proteus- UTIs vibrio-cholera haemophilus- meningitis in children bacterioides- appendicitis
what have a waxy mycolic acid in cell wall?
mycobacterium- TB, leprosy, buruli ulcer
what bacteria have NO cell wall?
mycoplasma- pneumonia
red- fried egg appearance
prokaryotic cells ribosomes?
70s
f-plasmids
fertility and conjugation
R-plasmids
resistance
describe plasmids
extrachromosomal DNA
found more in gram -
what is a dormant form of bacteria cell, most resistant?
endospore
which glycocalyx is tightly adhered
capsule
which glyocalyx is loosely adhered?
slime layer