MHC class

Question 1

MHC gene location

Answer 1

Chromosome 6, short arm, band 21

Question 2

MHC function

Answer 2

Controls tissue compatibility

Question 3

HLA in humans

Answer 3

Human Leukocyte Antigen complex

Question 4

MHC Class I antigen presentation

Answer 4

Endogenous antigens to CD8+ T-cells

Question 5

Highest MHC Class I expression

Answer 5

Lymphocytes and myeloid cells

Question 6

Low MHC Class I expression

Answer 6

Liver hepatocytes, muscle cells, sperm

Question 7

MHC Class I genetic loci

Answer 7

HLA A, B, C

Question 8

MHC Class I structure

Answer 8

Alpha chain, beta-2 microglobulin

Question 9

MHC Class II restriction

Answer 9

Immunocompetent immune cells

Question 10

MHC Class II antigen presentation

Answer 10

Exogenous antigens to CD4+ T-cells

Question 11

MHC Class II genetic loci

Answer 11

HLA-DP, DQ, DR

Question 12

MHC Class II structure

Answer 12

Alpha chain, beta chain

Question 13

MHC Class II expression

Answer 13

B cells, T cells (Class I only)

Question 14

MHC Class III

Answer 14

Involves complement proteins

Question 15

Complement proteins in MHC III

Answer 15

C2, C4a, C4b, Factor B, TNF

Question 16

Class I MHC function

Answer 16

Watchdog for viral, tumor, parasitic antigens

Question 17

Class II MHC function

Answer 17

Mount immune response to bacteria or external pathogens

Question 18

HLA Typing purpose

Answer 18

Identify HLA antigens/genes in transplant candidates/donors

Question 19

HLA Class I Typing

Answer 19

T and B lymphocytes

Question 20

HLA Class II Typing

Answer 20

B lymphocytes

Question 21

HLA Phenotyping principle

Answer 21

Complement dependent cytotoxicity

Question 22

HLA Phenotyping steps

Answer 22

Add antisera to lymphocytes, incubate, add complement, observe lysis

Question 23

HLA Phenotyping grading

Answer 23

Grade 1: 0-10% (negative), Grade 8: 81-100% (strong positive)

Question 24

Colored result in HLA Phenotyping

Answer 24

Dead cells (lysis)

Question 25

Colorless result in HLA Phenotyping

Answer 25

Live cells (no lysis, negative)

Question 26

HLA Genotyping principle

Answer 26

PCR-based amplification

Question 27

Importance of HLA typing for organ transplant

Answer 27

Tissue/organ transplant

Question 28

Immunosuppressive agent that blocks production and secretion of cytokines

Answer 28

Corticosteroids

Question 29

Immunosuppressive agent that interferes with lymphocyte maturation

Answer 29

Antimetabolites

Question 30

Immunosuppressive agent that blocks signal transduction in T lymphocytes

Answer 30

Calcineurin inhibitors

Question 31

Immunosuppressive monoclonal antibody binding CD25 receptor

Answer 31

Basiliximab and daclizumab

Question 32

Immunosuppressive monoclonal antibody binding CD52 receptor

Answer 32

Alemtuzumab

Question 33

Polyclonal antibody that depletes lymphocytes from circulation

Answer 33

Thymoglobulin