Antinuclear antibodies

Question 1

Immunofluorescent pattern for anti-dsDNA

Answer 1

Peripheral or homogeneous

Question 2

Disease associated with anti-dsDNA

Answer 2

SLE

Question 3

Immunofluorescent pattern for anti-ssDNA

Answer 3

Not detected on routine screen

Question 4

Disease associated with anti-ssDNA

Answer 4

SLE and other diseases

Question 5

Immunofluorescent pattern for anti-histone

Answer 5

Not specified

Question 6

Disease associated with anti-histone

Answer 6

Drug-induced SLE and other diseases

Question 7

Immunofluorescent pattern for anti-Sm, anti-RNP

Answer 7

Coarse speckled

Question 8

Disease associated with anti-Sm, anti-RNP

Answer 8

SLE and mixed connective tissue disease

Question 9

Immunofluorescent pattern for anti-SS-A/Ro, anti-SS-B/La

Answer 9

Finely speckled

Question 10

Disease associated with anti-SS-A/Ro, anti-SS-B/La

Answer 10

SLE and Sjögren’s syndrome

Question 11

Immunofluorescent pattern for antinucleolar antibodies

Answer 11

Prominent staining of nucleoli (smooth, clumpy, or speckled)

Question 12

Disease associated with antinucleolar antibodies

Answer 12

SLE and systemic sclerosis

Question 13

Immunofluorescent pattern for anti-Scl-70

Answer 13

Atypical speckled

Question 14

Disease associated with anti-Scl-70

Answer 14

Systemic sclerosis and scleroderma

Question 15

Immunofluorescent pattern for anti-Jo-1

Answer 15

Fine cytoplasmic speckling

Question 16

Disease associated with anti-Jo-1

Answer 16

Polymyositis and other diseases

Question 17

Immunofluorescent pattern for autoantibody-anticentromere

Answer 17

Discrete speckled

Question 18

Disease associated with autoantibody-anticentromere

Answer 18

CREST syndrome

Question 19

Features of CREST syndrome

Answer 19

Calcinosis, Raynaud’s phenomenon, esophageal dysmotility, sclerodactyly, telangiectasia

Question 20

Fluorescent staining pattern seen in RA, drug-induced SLE, Sjogren’s syndrome & MCTD

Answer 20

Homogenous (solid, diffuse)

Question 21

Fluorescent staining pattern associated with SLE in active stage and Sjogren’s disease

Answer 21

Peripheral/Rim (Ring, membranous)

Question 22

Fluorescent staining pattern with discrete fluorescent specks throughout the nucleus

Answer 22

Speckled (mottled, ‘pepper-dots’)

Question 23

Fluorescent staining pattern associated with scleroderma, Sjogren’s, SLE and Raynaud’s phenomenon

Answer 23

Nucleolar

Question 24

Fluorescent pattern for CREST variant of scleroderma

Answer 24

Centromere (Numerous discrete speckles)

Question 25

Method that uses polystyrene microspheres coated with individual nuclear antigens or HEp-2 extract

Answer 25

Microsphere Multiplex Immunoassay (MIA)

Question 26

Fluorescent marker used to detect antibody binding in MIA

Answer 26

Phycoerythrin-labeled anti-human IgG

Question 27

Instrument used to analyze the bead suspension and detect fluorescence in MIA

Answer 27

Flow cytometer with two lasers

Question 28

Method used to detect antibodies to double-stranded DNA (dsDNA)

Answer 28

Immunofluorescence using Crithidia luciliae

Question 29

Structure in trypanosomes used in immunofluorescence to detect dsDNA antibodies

Answer 29

Kinetoplast

Question 30

Test used to determine the immunologic specificity of a positive FANA test

Answer 30

Ouchterlony test

Question 31

Antibodies that bind to phospholipids or phospholipids complexed with protein

Answer 31

Antiphospholipid Antibodies

Question 32

Test results produced by Lupus Anticoagulant

Answer 32

Prolonged APTT and PT

Question 33

Antibody specific to lupus, first described in a patient named Smith

Answer 33

Anti-Sm antibody

Question 34

Family of small nuclear proteins associated with uridine-rich RNA

Answer 34

Extractable Nuclear Antigens (ENA)

Question 35

Most specific antibodies for SLE, diagnostic for SLE

Answer 35

Double-stranded DNA (ds-DNA) antibodies