Antinuclear antibodies

Question 1

Immunofluorescent pattern for anti-dsDNA

Answer 1

Peripheral or homogeneous

associated with SLE

Question 2

Immunofluorescent pattern for anti-ssDNA

Answer 2

Not detected on routine screen

Question 3

Disease associated with anti-ssDNA

Answer 3

SLE and other diseases

Question 4

Disease associated with anti-histone

Answer 4

Drug-induced SLE and other diseases

Question 5

Immunofluorescent pattern for anti-Sm, anti-RNP

Answer 5

Coarse speckled

associated with SLE and mixed connective tissue disease

Question 6

Immunofluorescent pattern for anti-SS-A/Ro, anti-SS-B/La

Answer 6

Finely speckled

associated wiht SLE and Sjögren’s syndrome

Question 7

Immunofluorescent pattern for antinucleolar antibodies

Answer 7

Prominent staining of nucleoli (smooth, clumpy, or speckled)

assoc with SLE and systemic sclerosis

Question 8

Immunofluorescent pattern for anti-Scl-70

Answer 8

Atypical speckled

associated with Systemic sclerosis and scleroderma

Question 9

Immunofluorescent pattern for anti-Jo-1

Answer 9

Fine cytoplasmic speckling

Associateed with Polymyositis and other diseases

Question 10

Immunofluorescent pattern for autoantibody-anticentromere

Answer 10

Discrete speckled

CREST syndrome

Question 11

Features of CREST syndrome

Answer 11

Calcinosis, Raynaud’s phenomenon, esophageal dysmotility, sclerodactyly, telangiectasia

Question 12

Fluorescent staining pattern seen in RA, drug-induced SLE, Sjogren’s syndrome & MCTD

Answer 12

Homogenous (solid, diffuse)

Question 13

Fluorescent staining pattern associated with SLE in active stage and Sjogren’s disease

Answer 13

Peripheral/Rim (Ring, membranous)

Question 14

Fluorescent staining pattern with discrete fluorescent specks throughout the nucleus

Answer 14

Speckled (mottled, ‘pepper-dots’)

Question 15

Fluorescent staining pattern associated with scleroderma, Sjogren’s, SLE and Raynaud’s phenomenon

Answer 15

Nucleolar

Question 16

Fluorescent pattern for CREST variant of scleroderma

Answer 16

Centromere (Numerous discrete speckles)

Question 17

Method that uses polystyrene microspheres coated with individual nuclear antigens or HEp-2 extract

Answer 17

Microsphere Multiplex Immunoassay (MIA)

Question 18

Fluorescent marker used to detect antibody binding in MIA

Answer 18

Phycoerythrin-labeled anti-human IgG

Question 19

Instrument used to analyze the bead suspension and detect fluorescence in MIA

Answer 19

Flow cytometer with two lasers

Question 20

Method used to detect antibodies to double-stranded DNA (dsDNA)

Answer 20

Immunofluorescence using Crithidia luciliae

Crithidia lucilae -flagellate parasite uses the housefly, M. domestica

Question 21

Structure in trypanosomes used in immunofluorescence to detect dsDNA antibodies

Answer 21

Kinetoplast

Question 22

Test used to determine the immunologic specificity of a positive FANA test

Answer 22

Ouchterlony test

Question 23

Antibodies that bind to phospholipids or phospholipids complexed with protein

Answer 23

Antiphospholipid Antibodies

Question 24

Test results produced by Lupus Anticoagulant in hematology

Answer 24

Prolonged APTT and PT

Question 25

Antibody specific to lupus, first described in a patient named Smith

Answer 25

Anti-Sm antibody

Question 26

Family of small nuclear proteins associated with uridine-rich RNA

Answer 26

Extractable Nuclear Antigens (ENA)

Question 27

Most specific antibodies for SLE, diagnostic for SLE

Answer 27

Double-stranded DNA (ds-DNA) antibodies