Lecture 11: Genetic/Molecular Approaches to Study Bacterial Infections Flashcards
____: An approach to the study of genes and their functions
Genomics
___: To characterize biological experiments carried out entirely on a computer
In silico
True or False: One can learn about gene content, dynamic, and organization all in silico (basic genomic information)
True
- important key to study evolution of bacterial pathogen
____ uses in silico process of assigning hypothetical function by homology, using species filter (subtracting all homologues of one known genome from another to define share and unshared genes)
A. Gene Content
B. Gene Organization
A. Gene Content
True or False: Gene Content is an approach that may help find genes responsible for certain phenotypes
True
Genes are organized into ___ or groups
clusters
What type of genomics technology provides the ability to conduct genome-wide surveys of genetic variation and differences in expression profiles of different strains?
A. Gene Content
B. Microarray
Microarray
Once genes and combination of genes are identified, it is essential to understand how the genes were ACQUIRED due to genome dynamics in bacteria, creating ____ ____ In bacterial populations
genetic diversity
What are the three main forces that shape bacterial genomes?
- gene change
- gene loss
- gene gain
What are the two main ways that pathogenic phenotypes can be acquired?
- horizontal transfer of existing genes
- de novo mutation
True or False: Comparative genomics can be used to identify, genus, species, subspecies
True
The broader the pan-genome, the more ___ the organisms
diverse
_____: the entire gene pool for that pathogen, includes genes not shared by all strains
A. Core-genome
B. Pan-genome
C. Strain specific genes
D. Dispensible genes
B. Pan-genome
_____: set of genes found across all strains of a pathogen species
A. Core-genome
B. Pan-genome
C. Strain specific genes
D. Dispensible genes
A. Core-genome
_____: found in more than one strain, but not in all strains
A. Core-genome
B. Pan-genome
C. Strain specific genes
D. Dispensible genes
D. Dispensible genes