Lecture 10

Question 1

____ and ____ are essential for loading helicase during Eukaryotic DNA replication.

Answer 1

CDC6 and CTD1

Question 2

____ is the polymerase that replicates the leading strand of the DNA, and ____ is recruited to increase its processivity.

Answer 2

DNA Pol epsilon does the leading strand, and PCNA is recruited to increase its processivity.

Question 3

____ replicates the Okazaki fragments of the ____ strand after ____ lays down the RNA primer.

Answer 3

Pol delta replicates the Okazaki fragments after Pol alpha lays the RNA primer.

Question 4

____ removes the RNA primer on the Okazaki fragments, ____ fills in the gaps, ____ removes the “flap,” and ____ “seals the deal.”

Answer 4

RNasH removes the primer, Pol delta fills in the gap, FEN1 removes the flap, and DNA ligase seals the deal.

Question 5

Meier-Gorlin Syndrome is characterized by mutation in the ____ recognition complex or in ____/____ which help load DNA helicase.

Answer 5

It is characterized by mutations in ORIGIN recognition complexes or CTD1/CDC6

Question 6

Seckel Syndrome is caused by mutations in ____, which aids during replication fork stalling.

Answer 6

It arises from mutations in ATR

Question 7

____ is the core catalytic unit of Telomerase and ____ serves as the RNA Template.

Answer 7

TERT is the core catalytic unit, and TERC serves as the RNA Template.

Question 8

Pulmonary Fibrosis, Aplastic Anemia, and Dyskeratosis Congenita are diseases that arise from defects in the different subunits of _____.

Answer 8

They all arise from defects in different subunits of TELOMERASE.