Lec 2.6 cytoskeleton 2 Flashcards
What is listeria?
pathogenic bac that invade your intestinal cells. Causing infection, food poisoning.
How do you treat listeria?
IV antibiotics
How does listeria work?
Enters and replicates in your intestinal cells. Smashing through organelles through the actin motility.
What are the listeria comet tails made up of?
Actin.
What are the 4 actin filament accessory proteins?
ARP complex, Formin, thymosin and profilin
What does the ARP complex do?
Nucleates assembly. forming weblike, highly branched chains. Remain associated with MINUS end.
What does Formin do?
Nucleats assembly of long UNbranched chains. Remains assocaited with PLUS end
What does Thymosin do?
Prevents assembly by binding to actin subunits
What does Profilin do?
Speeds elongation by binding to actin subunits
Which of the 4 actin filament accessory proteins allows actin filaments to make up the comet tails of Listeria?
ARP complex
How does ARP complex work most efficiently?
When it is bound to the side of a preexisting actin filament.
What pushes the Listeria bacteria along in the body?
Addition of actin branched filaments. Force pushes.
What does the protein cofilin do?
Makes branched actin disassemble. DISSAMBLES
what does the protein Gelsolin do?
Severs actin filaments and binds to PLUS end. DISSAMBLES
What does Tropomodulin do?
Prevents assembly/disassembly at MINUS end. STABALIZES
What does Tropomyosin do?
Prevents binding with other proteins. STABALIZES
What does the capping protein do?
Prevents assembly/disassembly at PLUS end. STABILIZES.
What are alpha actinin and Fimbrin?
Bundling proteins.
What does Filamin do?
Forms gel like structures
What does Spectrin do?
Attaches cytoskeleton to membrane
What does the ERM family do?
Attaches cytoskeleton to membrane
What is defective in HS?
Spectrin
What does Stathmin do?
Binds subunits, prevents assembly. MICROTUBULE
What does y-TuRC do?
nucleates assembly and remaind associated with MINUS end. Microtubule
Microtubules are nuceated from what specific location?
MTOC
What does Katanin do?
Severs microtubules
What do MAPS do?
Stabilizes tubules by binding along sides
What does XMAP215 do?
Stabilizes PLUS ends and accelerates assembly. microtubule
What does Kinesin 13 do?
Enhances catastrophic disassembly at PLUS end. Microtubule.
What does plectin do?
Cross-link protein, links microtubules to intermediate filaments
What does Tau (map protein) and MAP2 do?
Cause bundling of microtubules
Differentiate profilin and thymosin.
Speeds elongation by binding to actin subunits vs Prevents assembly by binding to actin subunits
