Lec 2.6 cytoskeleton 2

Question 1

What is listeria?

Answer 1

pathogenic bac that invade your intestinal cells. Causing infection, food poisoning.

Question 2

How do you treat listeria?

Answer 2

IV antibiotics

Question 3

How does listeria work?

Answer 3

Enters and replicates in your intestinal cells. Smashing through organelles through the actin motility.

Question 4

What are the listeria comet tails made up of?

Answer 4

Actin.

Question 5

What are the 4 actin filament accessory proteins?

Answer 5

ARP complex, Formin, thymosin and profilin

Question 6

What does the ARP complex do?

Answer 6

Nucleates assembly. forming weblike, highly branched chains. Remain associated with MINUS end.

Question 7

What does Formin do?

Answer 7

Nucleats assembly of long UNbranched chains. Remains assocaited with PLUS end

Question 8

What does Thymosin do?

Answer 8

Prevents assembly by binding to actin subunits

Question 9

What does Profilin do?

Answer 9

Speeds elongation by binding to actin subunits

Question 10

Which of the 4 actin filament accessory proteins allows actin filaments to make up the comet tails of Listeria?

Answer 10

ARP complex

Question 11

How does ARP complex work most efficiently?

Answer 11

When it is bound to the side of a preexisting actin filament.

Question 12

What pushes the Listeria bacteria along in the body?

Answer 12

Addition of actin branched filaments. Force pushes.

Question 13

What does the protein cofilin do?

Answer 13

Makes branched actin disassemble. DISSAMBLES

Question 14

what does the protein Gelsolin do?

Answer 14

Severs actin filaments and binds to PLUS end. DISSAMBLES

Question 15

What does Tropomodulin do?

Answer 15

Prevents assembly/disassembly at MINUS end. STABALIZES

Question 16

What does Tropomyosin do?

Answer 16

Prevents binding with other proteins. STABALIZES

Question 17

What does the capping protein do?

Answer 17

Prevents assembly/disassembly at PLUS end. STABILIZES.

Question 18

What are alpha actinin and Fimbrin?

Answer 18

Bundling proteins.

Question 19

What does Filamin do?

Answer 19

Forms gel like structures

Question 20

What does Spectrin do?

Answer 20

Attaches cytoskeleton to membrane

Question 21

What does the ERM family do?

Answer 21

Attaches cytoskeleton to membrane

Question 22

What is defective in HS?

Answer 22

Spectrin

Question 23

What does Stathmin do?

Answer 23

Binds subunits, prevents assembly. MICROTUBULE

Question 24

What does y-TuRC do?

Answer 24

nucleates assembly and remaind associated with MINUS end. Microtubule

Question 25

Microtubules are nuceated from what specific location?

Answer 25

MTOC

Question 26

What does Katanin do?

Answer 26

Severs microtubules

Question 27

What do MAPS do?

Answer 27

Stabilizes tubules by binding along sides

Question 28

What does XMAP215 do?

Answer 28

Stabilizes PLUS ends and accelerates assembly. microtubule

Question 29

What does Kinesin 13 do?

Answer 29

Enhances catastrophic disassembly at PLUS end. Microtubule.

Question 30

What does plectin do?

Answer 30

Cross-link protein, links microtubules to intermediate filaments

Question 31

What does Tau (map protein) and MAP2 do?

Answer 31

Cause bundling of microtubules

Question 32

Differentiate profilin and thymosin.

Answer 32

Speeds elongation by binding to actin subunits vs Prevents assembly by binding to actin subunits