3.12 molec biology methods review

Question 1

Cell culture conditions are what for each type of cell?

Answer 1

unique

Question 2

Describe what property of a protein is separated by each of the 4 types of column chromatography

Answer 2

Affinity chromatography separates protein by itself. Ion exchange chromatography separates proteins by charge, Hydrophobic interaction chroma separates by charge. Size exclusion chromatography separates by size.

Question 3

Describe the role of antibodies in blood typing and immunotherapy

Answer 3

When blood typing the interaction of antigen and antibody causes agglutination. . To prevent this mothers are given RhroGAM.

Question 4

Describe how ELISA works

Answer 4

an antibody binds an antigen of interest and produces a detectable signal.

Question 5

Know what is detected by Northern, southern and western blots

Answer 5

Northern: RNA. Western: proteins. Southern DNA.

Question 6

What is the sandwhich ELISA?

Answer 6

Both antibodies bund the antigen, the primary anitbody binds to the antigen and carries the signal

Question 7

what is the competition/inhibition ELISA?

Answer 7

Antigen presence is indicated by an absence or decrease of signal.

Question 8

What is the sandwhich ELISA?

Answer 8

Both antibodies bind the antigen, the primary anitbody binds to the antigen and carries the signal

Question 9

what is the competition/inhibition ELISA?

Answer 9

Antigen presence is indicated by an absence or decrease of signal.

Question 10

What treats HER2+ breast cancers?

Answer 10

Herceptin and trastuzumab