Lab Week 4 (Exam 2)

Question 1

What 2 basic capabilities should a microscope have?

Answer 1

1) magnification

2) resolution or resolving power

Question 2

What is magnification?

Answer 2

How the big the image can become

Question 3

What is resolution or resolving power?

Answer 3

How sharp the image is

Question 4

When you increase magnification what 3 things happen?

Answer 4

1) image darkens
2) field of view decreases
3) depth of field reduces

Question 5

What happens when the field of view decreases?

Answer 5

How much you can see all at once

Question 6

What happens when the depth of field reduces?

Answer 6

How much you can see sharply at one time without adjustment

Question 7

What 3 things can you do to correct the light source when the image is too dark?

Answer 7

1) turn up the lamp
2) system of lenses under stage and raise the condenser
3) in condenser there is iris diagram

Question 8

What is a stage?

Answer 8

Platform where slide sits

Question 9

What is the condenser?

Answer 9

System of lenses under the stage

Question 10

What is an iris diaphragm?

Answer 10

It is a whole where the light passes through

Narrow it down to get a brighter image

Question 11

What are the 2 lenses a microscope has?

Answer 11

1) eye piece or ocular (the place where you put your eyes)

2) nosepiece or turret (rotating piece above the stage

Question 12

What is the objective lens?

Answer 12

All lenses on rotating piece (nosepiece or turret)

Question 13

To figure out how magnified an image is what do you do?

Answer 13

Eyepiece x objective

Question 14

What is high dry magnification and how much is that?

Answer 14

Highest you can get without using oil

400x

Question 15

What is oil immersion lens and how much is it?

Answer 15

When you put the lens in oil to open light

Gives you the highest magnification

1000x

Question 16

Where is the base of a microscope?

Answer 16

Bottom of microscope

Question 17

Where is the arm of a microscope?

Answer 17

Side of microscope where you carry it

Question 18

Where and what is the lamp?

Answer 18

Under stage

Gives light

Question 19

What is the mechanical stage?

Answer 19

Wheels operating to position slide

Question 20

What is the coarse adjustment?

Answer 20

Moves stage up and down and used to focus image with lower magnification

Question 21

What is the fine or focus adjustment?

Answer 21

Moves stage up and down very little and is used to focus image with a higher magnification

Question 22

What is a stain?

Answer 22

Technique used to enable better visualization of cells and cell parts under the microscope