Lab Experiments Flashcards
What happened during the filtration experiment with calcium carbonate?
The calcium carbonate (chalk dust) is too big to go through the filter so the chalk stays behind and only the water goes through
Resulting in clear water
Force used is gravity
What happened in the diffusion experiment with the potassium permanganate?
The potassium permanganate dissolved in the water and becomes purple
And starts diffusing through the water in a circle mikaella motion becomes it goes down the concentration gradient
What happens in the osmometer experiment?
The water goes into the sucrose solution sac and up the thistle tube because of the concentration gradient
What happens in the hemolysis experiment?
In vials 1-4 the environment is hypotonic and the blood hemolysis (you can see through them)
In vial 5 the environment is isotonic (that’s why it’s blurry)
As water was less and less present in the tubes it became clear to cloudy
What happened in the dialysis experiment in bowl A with Lugols solution?
Diffusion through semipermeable membrane
Lugols molecules are small enough to go through the baggy
So when Lugols hit the starch it reacted and the baggy became blue black
Starch is too big to leave the baggy so the water with Lugols remained orange
What happened in the dialysis experiment in bowl B with silver nitrate?
Silver nitrate is too big to leave the baggy
The salt is small enough to go through the baggy and hit the water
When the Cl of the NaCl hits the silver nitrate outside of the baggy it turns white and cloudy
Petit with water and lugols solution is what color?
And what happens when there is starch present?
Orange/ yellow
Black/ blue
Petit with water and silver nitrate is what color?
And what happens when there is salt present?
Clear
White and cloudy
What happened in the digestion of the cream/ litmus with lipase experiment?
Tube 1 with distilled water and cream didn’t react because the enzyme wasn’t added and stayed blue
Tube 2 with lipase and cream turned red because when you break down fat you’re left with glycerol and fatty acids and the acids are red because they’re acid
What happened in the starch digestion with Benedict’s solution and with amylase experiment?
Tube 1 with starch and glucose solution became orange
Tube 2 with starch solution remained blue (no sugar)
Tube 3 with glucose solution became red (a lot of sugar in it)
What happened in the optimal pH solution with amylase experiment?
The pH 7 eventually turned orange because it was the optimal pH for the enzyme amylase to work and digest the starch
pH 4 was too acidic so amylase didn’t work and the color remained blue black
pH 9 was too basic so amylase didn’t work and the color remained blue black
What happened in the optimal temperature solution with amylase experiment?
The temp of 37C was the optimal temp for the amylase to work because it digested all the starch and the color went back to orange
The temp of 0C was too cold and the color remained blue black with the amylase not working but when the temp went back to room temp the amylase worked and digested the starch turning the color back to orange (because of the flexibility of the amylase was restored)
The temp of 90C was too hot and denatured the amylase so it didn’t work and the color remained blue black
