Lab 1 - Haematology past questions Flashcards
What are the effects of heparin?
It prevents blood from coagulating by catalysing the thrombin-antithrombin reaction
What is the function of methanol in fixation?
Fix the smear and precipitate the proteins
Buffy coat, explain
White blood cells have lower density than red, and will form thin layer on the top after centrifugation
How do you obtain blood serum
Centrifuge blood sample where fibrinogen is changed into fibrin by shaking in flask containing small glass beads
How do you get Defirbrinated blood?
Shake flask with blood and small glass beads to change fibrinogen into fibrin
What happens with the hematocrit level while increasing the epinephrine level?
It will increase
Give examples of anticoagulant agents.
Sodium Citrate
EDTA: Ethylene Diamine Tetracetic Acid
How do you perform the Lee white method?
Tilt blood in tube in intervals in 3 minutes to see if it coagulates
What is the Definition of hematocrit
Ratio of red blood cells to the total blood volume
Venous hematocrit is larger than arterial, explain why?
Because of ion transport and water flux associated with the CO2 transport
How does Increased erythrocyte sedimentation rate happen?
By displacing the albumin, the surface charge decrease
Describe the neutrophil granulocytes (Mammals )
Nucleus:
- Beanshape (young),
- Elongated (middleage)
- Segmented (mature),
Cytoplasm:
- Light pink cytoplasm,
Granules:
- Different granules
- 10 μm diameter
Enzymes:
- Lysozyme
- Myeloperoxidase
Describe the hetrophil granulocytes (birds)
Nucleus:
- Seveeral segments
Cytoplasm:
- Pink cytoplasm
Granules:
- Pale red granules)
Describe the eosinophil granulocytes
Nucleus:
- Segmented (usually bi-lobed)
Granules:
- Large bright red granules
- 14 μm diameter
Enzmes:
- Histaminase,
- Acid & alkaine phosphatase
- Eosinphil basic & cationic protein
Describe the basophil granulocytes
Nucleus:
- Hardly segmented
Granules
- Large blue granules,
- 15 μm diameter
Enzymes:
- Protease
