Genetics Flashcards
5 features of genetic information that have to be true for it to be able to work?
Stable for storage over time
Be able to faithfully replicated (semi conservative replication)
Be able to control expression of traits ( encode the sequence of proteins)
Be able to change in a controlled way
But also available for direct cellular processes
DNA has the structure of? Therefore leading to the creation of?
Double helix
Minor grooves and major grooves
What is a nucleotide?
Polymer made up of the repeating units of a sugar a base and a phosphate
Phosphate binds to sugar, which binds to base
Sugar + base =?
Nucleoside
Deoxyribose + adenine =?
Deoxyadenosine
Deoxyribose + guanine?
guanosine
Deoxyribose + cytosine?
cytidine
Deoxyribose + thymine?
thymidine
How do you know which end is the 5’ end and which is the 3’ end?
The 5’ end which have carbon 5 (the chimney) bonded to a phosphate group
The end will have an OH group on carbon 3
What is the directionality of a DNA strand?
5’ to 3’
DNA strands are anti parallel in a double stranded structure, what does this mean?
They are going in opposite directions (still going 5’ to 3’)
What holds DNA strands together?
Hydrogen bonds between complementary base pairs
Adenine to thymine
Cytosine to guanine
What’s a 2 carbon ring base called and what are the examples?
Purine
Adenine and Guanine
What’s a single carbon ring called, and what are the examples?
Pyrimidine
Cytosine and thymine
A purine will always bind to a?
Pyrimidine
How many hydrogen bonds between Adenine and thymine?
2
How many hydrogen bonds between Guanine and cytosine?
3
Why is the minor and major groove created?
Base pairs are planar
Stack?
Hydrophobic interactions
What type of double helix is DNA?
Right handed, (put left thumb up, direction of fingers shows the right hand direction)
Genetic information is carried in?
The sequence of bases in DNA = digital code
How does the structure of DNA provide a mechanism for heredity?
You can control the expression of traits (encode the sequence of proteins)
Be able to change in a controlled way
What is the genome?
All the DNA in the nucleus of a cell (ie. the complete set of genes of an organsism)
What’s special about mitochondria and chloroplasts in terms of DNA?
They have their own small circular genomes
Nuclear genetic material is distributed among discrete units called?
Chromosomes
How are nucleosomes formed?
DNA wraps twice around 8 core histone proteins to form nucleosomes
How are chromatin fibres formed?
Histon H1 brings the nucleosomes together
How is chromatin fibre folded into loops (part of the chromosome)?
Chromatin fibres are further condensed by scaffold proteins
What is the net result of creating chromatin fibre which then forms the chromosome?
Each DNA molecule has been packaged into a mitotic chromosome that is 10,000 fold shorter than it’s fully extended length
2 types of chromatin?
euchromatin
heterochromatin
Features of euchromatin?
Less compact DNA form
Contains genes that are frequently expressed
Features of heterochromatin?
More compact
Contains DNA that is not transcribed
What are nucleoli?
Regions where chromosomes carrying rRNA cluster, rRNAs are synthesised and ribsomes are assembled
Chromosomes organise themselves into discrete patches called?
Chromosome territories
Where are active genes normally located?
In the euchromatic region of the chromosome, and near the chromosomes territory boundary
What does transcription do very basically?
Prodcues an RNA molecule that is complementary to one strand of DNA
DIfferences in RNA compared to DNA?
RNA has the sugar ribose instead of deoxyribose
Has the base uracil instead of thymine
What do differences in RNA mean?
RNA is More chemically reactive than DNA as RIbose has 2’ OH group (so less stable so only used for short term storage of information, whereas DNA is long term)
RNA is more prone to mutate than DNA, as cytosine deamination to uracil can’t be detected and repaired in RNA
RNA is single stranded and not double, meaning there is no backup of genetic information (but it can still base pair with itself)
What enzyme helps synthesise RNA and describe its structure?
RNA polymerase, Has area where the short region of DNA has been separated, and a ribonucleoside triphosphate uptake channel allows the ribonucleosides to come in
What direction is RNA synthesised?
5’ to 3’ direction (so DNA will be read 3’ to 5’)
How is energy provided for the formation of a phosphodiester bond when synthesising RNA?
Breakage of phosphoanhydride bond of NTP (the phosphate chain in the nucleoside triphosphate, 2 are lost and the other binds to the sugar backbone with the base attached)
What does mRNA do?
Messenger RNA
Code for proteins
What does rRNA do?
Ribosomal RNA
Forms the basic structure of the ribosome and catalyze protein synthesis
What do tRNA’s do?
Transfer RNA, central to protein synthesis as are adaptors between mRNA and amino acids
What is the transcriptome?
Set of all RNA molecules
Varies on the cell (different genomes)
What is the central dogma?
Information flow from DNA to RNA via transcription then from RNA to Protein via translation
How is mRNA processed before it leaves the nucleus?
The 5’ end of the mRNA gets “capped” with an atypical nucleotide
The 3’ end of the mRNA gets a tail of poly-A nucleotides
Introns (non coding sequences) have to be removed from the mRNA
One way in which a bacterial gene is different to a eukaryotic gene?
In a bacterial gene all the gene is a protein coding region (exon)
In a eukaryotic gene there are protein coding regions (exons), but also non-coding regions (introns)
Describe briefly the splicing process that occurs in pre mRNA to remove introns?
3’ clevage and addition of a poly(A) tail
Intron excision occurs by snRNA’s that bind to proteins to form snRNP’s, which form the core of the splicesosome
The splicesosome causes the intron to bend and one side which break and bind to itself forming a intron lariat
Exon ligation then occurs to create the mRNA reading for translation
What can alternative splicing lead to?
More than one gene being expressed from one gene
Describe mRNA’s keaving the nucleus?
RNA binding proteins mark a mature mRNA for export from the nucleus
Leaves through nuclear pore
What determines how much protein is translated by a gene relating to the mRNA?
The stability of the mRNA, as it’s being degraded by the cell
So more stable it is the more times it can be translated
How many types of amino acids?
20
How do we know that genetic code isn’t read overlapping?
Single base mutations only affect one amino acid
What is proflavin?
Planar molecule that intercalates between base pairs in DNA
Causes frameshift mutations (insertion of an extra base, or deletion of a base)
What is the genetic code of mRNA read in?
Sets of 3 nucleotides called codons
Some amino acids have more than one tRNA, meaning they are?
degenerate
The genetic code is universal, meaning?
It’s found in all organisms, however there are variations in mitochondria
Very bsaically what do mRNA, tRNA and rRNA do in translation?
mRNA carries the genetic information
tRNA deciphers the codons of the mRNA
rRNA makes up the ribosome