Evolution and Genetic Engineering Flashcards

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1
Q

fathers of evolution

A

charles darwin

alfred wallace

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2
Q

observations of the theory of natural section

A

overbreeding: organisms produce a large number of offspring

population numbers stay constant: numbers reach the max. the environment can support

inherited variations occur: no two members of the same species ever look alike (except identical twins)

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3
Q

conclusions of the theory of natural selection

A

struggle for existence: overbreeding leads to competition within species for food, water, mates

survival of the fittest: variation occurs within species and the better adapted members are more likely to survive and leave more offspring
these survivors will pass on their better genes to their offspring, who will also show this beneficial variation

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4
Q

natural selection

A

variation occurs within species and members of a species with genetic traits that allow them to be better adapted to survive in their environment will reproduce and pass these on to following generations ie survival of the fittest

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5
Q

evolution

A

inheritable change within a population in response to changes in the environment or over time, giving rise to new species

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6
Q

evidence of evolution: source

A

the study of fossils

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7
Q

fossils as evidence of evolution

A

can be aged and compared on a timescale
can be compared with modern organisms
can be linked to environmental change

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8
Q

example of a fossil use

A

the anatomy of a horse

size increased over time from 0.4m 60 million years ago to 1.6m today

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9
Q

stages of genetic engineering

A
isolation
cutting
ligation
cloning
expression
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10
Q

isolation (GE)

A

target gene from donor is identified and removed

plasmid DNA from bacterium removed (acts as a vector)

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11
Q

cutting (GE)

A

DNA and plasmid cut at specific sites using restriction enzymes

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12
Q

ligation

A

target gene inserted into plasmid using DNA ligase to join it together = recombinant DNA
transformation: recombinant DNA introduced into bacterial cell
introduction of base sequence changes: the sequence of bases in the host is now different

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13
Q

cloning (GE)

A

bacteria reproduce asexually by binary fission, produce many identical copies of themselves containing recombinant DNA

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14
Q

expression (GE)

A

the gene causes the production of a protein (product)

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15
Q

application of GE in plant

A

herbicide resistant crops
inserting a bacteria gene for herbicide resistance in crop plants, so that when the herbicide is sprayed it will kill weeds not plant

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16
Q

application of GE in animals

A

blood clotting factor
a human gene for this protein has been inserted into sheep DNA and they can produce the protein in their milk to treat haemophilia

17
Q

application of GE in micro-organisms

A

bacteria make insulin
inserting the gene for human insulin into a bacterium which then produces human insulin for use as injections by diabetics