chapter six part one Flashcards
components of microscopy
visible light, magnification, resolution, wavelength, contrast
light
light passed through specimen then through glass lenses
magnification
ratio of object’s image size to real size
resolution
measure of clarity of image, ability to see 2 distinct things
wavelength
light/electrons
contrast
difference in brightness between light/dark areas
stain
cell components stand out, can be distinguished
condenser
below stage, focuses light on specimen
when was microscopy first invented?
1590
Robert Hooke and cells
- 1665
- dead cells of oak bark
2 types of electron microscopes
transmission and scanning
electron microscope
focuses beam of electrons through specimen and on surface
TEM
internal structure (organelles), specimen stained
organelles
membrane-enclosed structures within eukaryotic cell
SEM
- detailed study of topography
- gold on surface, electrons excited, translated to pattern on screen
cell fractionation
takes cells apart, separates major organelles and structures
- centrifuges
centrifuges
holds mixtures of disrupted cells at different speeds, components settle in bottom of tube
2 types of cells
prokaryota and eukaryota
prokaryotic cells
- domain bacteria and archaea
- DNA in nucleoid (no membrane)
- no membrane-bound organelles
- smaller
eukaryotic cells
- domain eukarya
- DNA in nucleus
- membrane-bound organelles
- larger
similarities between prokaryotic and eukaryotic
plasma membrane, cytosol, chromosomes, ribosomes