Chapter 9 pt 1 Flashcards
what does the genome do
determine where the organism will live, what it can do, antibiotic resistance and pathogenesis etc
mitochondria DNA
circular chromosome
genome of cell
DNA
genome
sum total of genetic material of a cell (chromosomes +mitochondria/chloroplasts and or plasmids)
genome of virus
DNA or RNA
DNA complexed with protein
chromosomes
fundamental unit of heredity
gene
three types of genes
structural genes (code for proteins), genes that code for RNA, genes that control gene expression (regulatory genes)
smallest virus
4 to 5 genes
ecoli chromosome
4,288 genes
-1mm
-1,000 x longer than cell
human cell
46 chromosomes
-31,000 genes
-6ft long
-180,000 x longer than cell
what wraps around chromosome
naked DNA
basic unit of DNA
nucleotide
nucleotide parts
-5c deoxyribose
-phosphate group
-nitrogenous base (AGCT)
how does the nucleotide bond to make sugar phosphate backbone?
covalent bond
-each sugar= 2 phosphates (5 carbon and 3 carbon)
a and t
2 h bonds
g and c
3 h bonds
replication occurs?
on both strands simultaneously
what ensures fidelity of replication?
semiconservative replication process
primase
starts process
where does replication begin?
at the origin of replication
where is rna primer synthesized
at the origin of replication by primase
DNA pol 3
add nucleotides in 5 to 3 direction
DNA pol 1
removes RNA priumers and replaces them with DNA
topoisomerase
prevents supercoiling
is ribosomal RNA shorter than mRNA and tRNA
NO
how many enzymes does replication require
30
what happens when the fork meets?
what do bacteria have to replicate faster?
two forks
-copy genome in half the time
gyrase
a type of topoisomerase to prevent supercoiling
how is information that is stored in DNA expressed
it is conveyed to RNA through transcription then translation for proteins
5 c
ribose in rna
is rna ss or ds
ss
mrna
sequence of amino acids in proteins
-carries mastercode to ribosome
-in codons
-NOT the final product on genbe expression
trna
bring amino acids to ribosome during translation
-exposes the anticodon that will complement complement mRNA
rRNA
ribosomal
-forms the major part of a ribosome and where protein synthesis happens
primer
an rna that primes dna for replication
where is tRNA
in the large ribosomal subunit
initiation of transcription
promoter binding
termination
release of transcript
what happens at the ribosome
all of the elements needed for protein synthesis are brought together
5 stages of protein synth
- initiation
- elongation
- termination (stop codon)
- protein folding
- post translational processing
every cell has
different ways to get over initiation
-this allow us to have different antibiotics
-target different things??
what happens if protein folding goes wrong
protein cant function properlyh
how many aa combinations
64