Chapter 9 pt 1 Flashcards

1
Q

what does the genome do

A

determine where the organism will live, what it can do, antibiotic resistance and pathogenesis etc

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2
Q

mitochondria DNA

A

circular chromosome

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3
Q

genome of cell

A

DNA

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4
Q

genome

A

sum total of genetic material of a cell (chromosomes +mitochondria/chloroplasts and or plasmids)

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5
Q

genome of virus

A

DNA or RNA

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6
Q

DNA complexed with protein

A

chromosomes

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7
Q

fundamental unit of heredity

A

gene

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8
Q

three types of genes

A

structural genes (code for proteins), genes that code for RNA, genes that control gene expression (regulatory genes)

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9
Q

smallest virus

A

4 to 5 genes

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10
Q

ecoli chromosome

A

4,288 genes
-1mm
-1,000 x longer than cell

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11
Q

human cell

A

46 chromosomes
-31,000 genes
-6ft long
-180,000 x longer than cell

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12
Q

what wraps around chromosome

A

naked DNA

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13
Q

basic unit of DNA

A

nucleotide

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14
Q

nucleotide parts

A

-5c deoxyribose
-phosphate group
-nitrogenous base (AGCT)

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15
Q

how does the nucleotide bond to make sugar phosphate backbone?

A

covalent bond
-each sugar= 2 phosphates (5 carbon and 3 carbon)

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16
Q

a and t

A

2 h bonds

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17
Q

g and c

A

3 h bonds

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18
Q

replication occurs?

A

on both strands simultaneously

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19
Q

what ensures fidelity of replication?

A

semiconservative replication process

20
Q

primase

A

starts process

21
Q

where does replication begin?

A

at the origin of replication

22
Q

where is rna primer synthesized

A

at the origin of replication by primase

23
Q

DNA pol 3

A

add nucleotides in 5 to 3 direction

24
Q

DNA pol 1

A

removes RNA priumers and replaces them with DNA

25
Q

topoisomerase

A

prevents supercoiling

26
Q

is ribosomal RNA shorter than mRNA and tRNA

A

NO

27
Q

how many enzymes does replication require

A

30

28
Q

what happens when the fork meets?

A
29
Q

what do bacteria have to replicate faster?

A

two forks
-copy genome in half the time

30
Q

gyrase

A

a type of topoisomerase to prevent supercoiling

31
Q

how is information that is stored in DNA expressed

A

it is conveyed to RNA through transcription then translation for proteins

32
Q

5 c

A

ribose in rna

33
Q

is rna ss or ds

A

ss

34
Q

mrna

A

sequence of amino acids in proteins
-carries mastercode to ribosome
-in codons
-NOT the final product on genbe expression

35
Q

trna

A

bring amino acids to ribosome during translation
-exposes the anticodon that will complement complement mRNA

36
Q

rRNA

A

ribosomal
-forms the major part of a ribosome and where protein synthesis happens

37
Q

primer

A

an rna that primes dna for replication

38
Q

where is tRNA

A

in the large ribosomal subunit

39
Q

initiation of transcription

A

promoter binding

40
Q

termination

A

release of transcript

41
Q

what happens at the ribosome

A

all of the elements needed for protein synthesis are brought together

42
Q

5 stages of protein synth

A
  1. initiation
  2. elongation
  3. termination (stop codon)
  4. protein folding
  5. post translational processing
43
Q

every cell has

A

different ways to get over initiation
-this allow us to have different antibiotics
-target different things??

44
Q

what happens if protein folding goes wrong

A

protein cant function properlyh

45
Q

how many aa combinations

A

64