chapter 11 pt 3

Question 1

where do listeria monocytogens replicate?

Answer 1

cytoplasm of cells after they induce phagocytosis to get in
-to avoid humoral immune system?

Question 2

what can listeria monocytogens cause?

Answer 2

neonatal listerosis or meneingitis

Question 3

how does listeria monocytogens get in?

Answer 3

intestine
-internal mediates attach to an enterocytle and eneter in an endocytotic vacuole

Question 4

how does listeria monocytogens escape from vacuole?

Answer 4

LLO
-listerolysin luses
-escapes into cytoplasm

Question 5

how does listeria monocytogens invade other cells?

Answer 5

cytoskeleton gets modified and it moves along fibers by polimerizing actin (comet tail)
-enters that cell in a double vacuole that gets lysed by LLO
-then can cause bloodstream invasion

Question 6

listerosis cases caused by

Answer 6

dairy, meat, poultry
-can contaminate food or grow in animal intestine
-can be mild or cause septicemia

Question 7

when is listeriosis worse?

Answer 7

in immunocompromised people

Question 8

treat listeria monocytogens

Answer 8

-ampicillan
-pasteurization
-cooking

Question 9

enterobacteriaceae

Answer 9

-facultative anaerobes
-ferment glucose
-reduce nitrate to nitrites
-oxidase negative and catalase positive
-coliforms and non coliforms

Question 10

coliform

Answer 10

lactose fermenter

Question 11

how to screen for enterobacteriaceae

Answer 11

selective and differential screening

Question 12

rapid assay for enterics

Answer 12

BBL enterotube 2

Question 13

basic mechanisms of diahrrea disease

Answer 13

-toxigenic
-invasive

Question 14

toxigenic

Answer 14

microbe in intestine, makes a toxin, diahrrea caused by fluid secretion

Question 15

invasive

Answer 15

bacteria invades cell, gets to blood and is killing

Question 16

most agents of diahrrea are not?

Answer 16

part of the normal microbiota population

Question 17

diahrrea makes up?

Answer 17

40% of the worlds infectious disease
18% of deaths

Question 18

gastroenteritis

Answer 18

diahrrea disease

Question 19

what triggers immune response and contributed to pathogenicity

Answer 19

surface antigens

Question 20

outermembrane LPS

Answer 20

-surface antigen
-recognition of repeating oligosaccharides on LPS molecule
-O Ag

Question 21

K antigen

Answer 21

cell surface polysaccharides
-capsule or slime layer
-v.f.

Question 22

H antigen

Answer 22

flagella

Question 23

what have fimbrae?

Answer 23

many enterobacteriaceae
-surface pili
-NOT antigenic proteins
-v.f.

Question 24

endotoxins and exotoxins are?

Answer 24

virulence factors

Question 25

most prevalent enteric bacillus

Answer 25

escherichia coli -in the gut -aerobic and non fastidious -150 distinct O antigens and many K and H antigens -some have plasmid and v.f. others are opportunists

Question 26

how are e coli differentiated

Answer 26

by surface features -makes them different stereotypes of e coli

Question 27

shiga toxin

Answer 27

(stx) -e.coli toxin/ v.f. -ab toxin that interferes with protein synthesis by disturbing rRNA binding to ribosome

Question 28

what gives ecoli specificity for target tissue

Answer 28

fimbrae -essential for some types of infection

Question 29

pathogenic strains of ecoli

Answer 29

-enterotoxigenic (ETEC) -enteroinvasice (EIEC) -enteropathogenic (EPEC) -enterohemorrhagic (EHEC)

Question 30

which is the most serious ecoli

Answer 30

-enterohemorrhagic -EHEC -carries shiga toxin gene from the prophage shigella

Question 31

which ecoli doesnt have LT and ST toxin

Answer 31

-EPEC -enteropathogenic -linked to wasting from infantile diarrhea

Question 32

which ecoli does not invade past the intestine

Answer 32

-EIEC -enteroinvasice -causes inflammation of large intestine -appears like shigella dysentary -epithelial tissue of the intestine

Question 33

most common cause of travelers diahhrea

Answer 33

-ETEC -enterotoxigenic -diahhrea due to LT and ST enterotoxins -has fimbrae for adherance

Question 34

greatest cause of mortality in babies

Answer 34

pathogenic ecoli causing infantile diahrrea -coliform count

Question 35

what can coliform count do?

Answer 35

show fecal contamination in water -ecoli is the proxy bc it is easy to -find ecoli= finding waste contamination of animal poop