Chapter 6 - B cell development (Exam 3)

Question 1

B cells develop in the

Answer 1

bone marrow

Question 2

B cells are activated in the

Answer 2

secondary lymph tissues

Question 3

B cells migrate from

Answer 3

bone marrow –> blood –> secondary lymph tissues

Question 4

Function of B cells

Answer 4

Make immunoglobulin

Question 5

Negative selection of B cells occurs in

Answer 5

bone marrow

Question 6

Positive selection of B cells occurs in

Answer 6

Secondary lymphoid tissues

Question 7

1st identifiable cell of B cell lineage

Answer 7

Pro-B cell

Question 8

Anti-CD34

Answer 8

Isolate development of B cells

Question 9

Rearrangement of heavy-chain genes begins where

Answer 9

in pro-b cell

Question 10

CD molecules

Answer 10

Cluster of differentiation
Define developmental stage and properties of cells

Question 11

Where mu heavy chain is made

Answer 11

Large pre-B cell

Question 12

1st checkpoint

Answer 12

Pro B cells
Rearrangement of heavy-chain genes

Question 13

2nd checkpoint

Answer 13

Pre B cells
Rearrangement of light-chain genes

Question 14

Stroma

Answer 14

Supportive cells and connective tissue in any organ

Question 15

Lymphoid Stromal cells provide

Answer 15

contact-mediated signaling
soluble growth factors (IL-7)

Question 16

Heavy chain rearrangement is inefficient and imprecise in

Answer 16

Pro-B cells

Question 17

Nonproductive rearrangement

Answer 17

P and N nucleotide insertion no longer codes for Ig

Question 18

Productive rearrangement

Answer 18

P and N nucleotide insertion codes for Ig

Question 19

Apoptosis in B cells occurs when

Answer 19

V-DJ rearrangement does not occur on either allele

Question 20

Allelic Exclusion

Answer 20

Only one allele for each heavy and light chain
Ensures single antigen specificity
(monoclonal antibody)

Question 21

Allelic exclusion at heavy chain gene

Answer 21

1 heavy chain with one light chain

Question 22

Without allelic exclusion at heavy chain gene

Answer 22

2 different heavy chains and 1 light chain
You will die

Question 23

Formation of Pre-B cell receptor signals

Answer 23

Successful heavy chain rearrangement

Question 24

Surrogate light chain

Answer 24

Signals pro B to pre B cell

Question 25

Functions of Pre-B cell receptors

Answer 25

Eliminates pro-B without functional mu heavy chain Prevents Ig from having more than 1 heavy chain

Question 26

Allelic exclusion in pre-b cell receptors

Answer 26

Stop RAG genes degrade RAG proteins Stabilize Ig gene arrangement

Question 27

After expansion of pre b cells

Answer 27

Make ~85 pre b cells with same heavy chain but different light chain

Question 28

Rearrangement of light chains

Answer 28

Allelic exclusion Isotype exclusion (k or l)

Question 29

When complete Ig is at surface

Answer 29

Signals to stop light-chain rearrangement and proliferate

Question 30

Pax5

Answer 30

binds to B cell promoters; defines B Required for activation of genes for rearrangement of Ig genes B cells ONLY

Question 31

Negative selection

Answer 31

against B cells that recognize self

Question 32

Non self reactive B cells

Answer 32

alternative splicing will make IgD and IgM then B cell leaves bone marrow

Question 33

Mechanisms of B cell tolerance

Answer 33

Central tolerance (in BM) Peripheral tolerance (out BM)

Question 34

In ALL immature B cells, ____ of any antigen to receptor causes ______.

Answer 34

binding apoptosis/inactivation

Question 35

Central tolerance

Answer 35

Receptor editing Anergy

Question 36

Receptor Editing

Answer 36

Repeatedly rearrange light chains and test for self-reactivity

Question 37

Self tolerant B cell

Answer 37

Leaves BM and goes to secondary lymphoid tissue to mature

Question 38

Clonal deletion

Answer 38

selective death of developing lymphocyte

Question 39

Anergy

Answer 39

developmental arrest where B cell makes IgM and IgD but IgM is nonfunctional and retained inside cell Cell enters periphery but dies after 1-5 days

Question 40

Central and peripheral tolerance do not remove

Answer 40

B cells reactive to inaccessible self antigens

Question 41

Autoimmune response

Answer 41

Self-antigens accessible to binding surface IgM

Question 42

Postive selection

Answer 42

Non-self antigen in B cell follicles of secondary lymphoid tissue promotes B cell survival

Question 43

B cells home to lymph node from blood through

Answer 43

HEV high endothelial venule B cells compete for access to follicles

Question 44

Follicular dendritic cells

Answer 44

B cell must interact to mature

Question 45

Mature B cells remain in

Answer 45

circulation to survive

Question 46

Naive B cell

Answer 46

recirculates through secondary lymphoid tissues until it recognizes antigen

Question 47

Anergic B cell

Answer 47

enters lymphoid tissue but excluded from follicles so they die

Question 48

B cell activation/differentiation is triggered by

Answer 48

binding of mature B cell surface Ig to antigen from pathogen

Question 49

B cell activation/differentiation leads to

Answer 49

B cell proliferation B cell differentiation SHM, isotype switching

Question 50

Mature B cells encountering antigen form

Answer 50

germinal centers

Question 51

Germinal centers

Answer 51

Affinity maturation Memory B cells

Question 52

Affinity maturation

Answer 52

only SHM and isotype switched B cells are selected for high affinity surface Ig and complete differentiation into plasma cells make high affinity Abs

Question 53

Memory B cells

Answer 53

Quiescent, permanent, high affinity and isotype switched Abs as immune response Easily stimulated

Question 54

B cell tumors arise from

Answer 54

a single transformed cell

Question 55

Multiple myeloma

Answer 55

In bone marrow Mutated Ig V gene