Chapter 20 and 21 Flashcards
A DNA molecule made into vitro with segments from different sources
Recombinant DNA
Manipulation of organisms or their components to produce useful products
Biotechnology
Direct manipulation of genes for practical purposes
Genetic engineering
Small, circular double-stranded DNA molecule that carries accessory genes separate from those of a bacterial chromosome
Plasmid
The production of multiple copies of a single gene
Gene cloning
DNA molecule that can carry foreign DNA into a host cell and replicate there
Cloning vector
Used for ampicillin resistance
ampR
Used for lactose breakdown
lacZ
A set of clones containing all the DNA segments from a genome, each with a plasmid, BAC, or other cloning vector
Genomic library
The process of base pairing between a gene and a complementary sequence on another nucleic acid molecule
Nucleic acid hybridization
Recognize and cut a specific DNA sequence
Restriction enzyme
Specific sequence of nucleotides at which a restriction enzyme cleaves a DNA molecule
Recognition sequence