Chapter 16 Flashcards
Change in genotype and phenotype due to the assimilation of external DNA
Transformation
what are the two chemical components of chromosomes
DNA and protein
What is the transformation factor?
DNA
How does a bacteriophage destroy a bacterial cell
It puts DNA into a cell and it grows and burst
What element is common in DNA?
Phosphorus
What are Chargaff’s rules?
Based composition varies between species and the number of adenine and Thymine are equal while the number of cystenine and guanine are equal
What are the three components of a nucleotide?
Phosphate, sugar, nitrogenous base
Who are the two men who built the first molecular model of DNA?
Watson and crick
Name two purines
Adenine and guanine
Name 2 pyrimidines
Cystenine and thymine
Subunits run in opposite directions
Anti-parallel
Two parental strands come back together during replication
Semi conservative model of replication
Short stretches of DNA having specific sequence of nucleotides
Origins of replication
Adds a new nucleotides to complementary strands
Leading strand
Away from replication fork
Lagging strand
Sacrament of DNA synthesized away from replication fork
Okazaki fragments
Untwist and separates strands
Helicases
Holds DNA strands apart
Single-strand binding proteins
Adds short RNA segments to which DNA polymerase III can attach nucleotides during replication
Primase
Add DNA nucleotides to new strands
DNA polymerase III
Removes RNA primer and replaces it with DNA
DNA polymerase I
Relieve strain caused by unwinding and joins DNA fragments together
Topoisomerase
DNA cutting
Nuclease
Join sugar phosphate to Okazaki fragments
Ligase
Reviews and repairs DNA
Repair enzymes
Linking of thymine bases which interfere with DNA replication
Thymine dimer
Adds segments to the ends of chromosomes to prevent shortening during replication
Telomerase
Eukaryotic chromatin that is condensed and is not transcribed
Heterochromatin
Less compacted chromatin and is available for transcription
Euchromatin
Recognizes splice sites and combines with proteins to form spliceosomes
snRNP
Editing complex containing “snurps” that removes introns and splices together exons
Spliceosomes
Region on DNA where RNA polymerase binds to start transcription
Promoter
Set the stage for the identity of the transforming molecule
Griffith
Showed the transforming molecule was DNA
Avery, MacLeod, and McCarty
Proved that DNA was protein
Hershey and Chase
Analyzed DNA with X-ray crystallography to determine the structure of DNA
Wilkins and Rosalind Franklin
DNA to mRNA
Transcription
mRNA to protein
Translation