Chapter 15 - Recombinant DNA Engineering

Question 0

procedures used to carry out genetic engineering

Answer 0

recombinant DNA technology

Question 1

deliberate modification of organism’s genetic information by directly changing the sequence of nucleic acids in its genome

Answer 1

genetic engineering

Question 2

generation of a large number of genetically identical dna molecules

Answer 2

cloning

Question 3

use of organisms to form useful products

Answer 3

biotechnology

Question 4

use of microbes to manufacture important compounds or the use of microbes as products in their own right

Answer 4

industrial microbiology

Question 5

prokaryote enzymes that recognize and bind specific sequnces in dna called recognition sites

Answer 5

restriction enzymes

Question 6

dna to be cloned

Answer 6

source/target dna

Question 7

plasmid, phage or other dna used to transport source dna into cell for cloning

Answer 7

vector

Question 8

cell into which the vector containing the source dna is inserted

Answer 8

host cell

Question 9

synthesized ds dna using ss mRNA from a gene as a template

Answer 9

cDNA (complementary)

Question 10

most common bacteria host cell

Answer 10

E. coli

Question 11

most common eukaryotic host cell

Answer 11

S. cerevisiae

Question 12

enables gene amplification to see if two types of DNA match and is a process used in crime when only a small amount of DNA is found

Answer 12

polymerase chain reaction

Question 13

thermostable DNA polymerase such as blank are required for PCR

Answer 13

Taq polymerase