Chapter 10 Flashcards
Taxonomy
Science of classifying organisms
Taxa
Taxonomic categories into which organisms are arranged that reflect degrees of relation among them
Bionomial nomenclature
Genus+specific epithet
Used worldwide
Culture
Grown in lab media
Clone
Population of cells derives from single cell
Strain
Genetically different cells within a clone
Serology
Combine known antiserum plus unknown bacterium
What is the importance of taxonomy?
The science of classifying organism.
Provides universal names for organisms.
Provides a reference for identifying organisms.
Major characteristics archae
Archae: contains no peptidoglycan, composed of branched carbon attached to glycerol by ether linkage.
Eukaryotic species
A group of closely related organisms that breed among themselves. Animalia, plantae, fungi, and protista.
Animalia
Multicellular, no cell wall, chemoheterotrophic
Plantae
Multicellular or unicellular. Chemoheterotrophic, cell walls of chitin, develop from spores or hyphal fragments.
Protista
A catchall kingdom for eukaryotic organisms that do not fit other kingdoms. Grouped into clades based on rRNA
Taxonomic hiererachy
Domain, kingdom, phylum, class, order, family, genus, species
Classification
Placing organisms in groups of related species.
Identification
Matching characteristics of an unknown organism to lists of known organism. Example: clinical lab identification
Morphological characteristics
Useful for identifying eukaryotes
Differential staining
Gram staining, acid fast staining
Biochemical tests
Determines presence of bacterial enzymes.
Linnaeus
introduced formal system of classification
slide aggulation test
show clumpping when mixed with antibodies
ELISA test
Enzyme-Linked Immunosorbent Assay- known antibodies, unknown type of bacteria with antibodies linked to enzyme
western blotting test
gel electrophoresis tests proteins by size
southern blotting test
uses nucleic acid hybridization to identify unknown microorganisms
Major characteristics Bacteria
Bacteria: contains peptidoglycan, composed of straight carbon chains attached to glycerol by ester linkage.
Major characteristics eukarya
Eukarya: contains carbohydrates composed of straight carbon chains attached to glycerol by ester linkage.